The metabolism of linoleate (18:2o>6) was studied in four chow-fed pregnant rhesus monkeys using 13C-18:2co6 and high precision mass spectrometry. Labeled linoleate (18:2(o6, 29 mg) in Intratipid carrier was administered intravenously via chrome indwelling maternal catheter at 151 days gestation. Maternal plasma was taken hourly after the dose. Five days post-dose fetuses were removed by Caesarian section, and fetal liver, brain, and retina fatty acids of the total Kpid extract were analyzed, m maternal plasma, 18:2ω6* decreased to about 60% of total label, while 18:3ω6, 20:3o>6, and arachidonic acid enrichments increased over the 5 d period. Labeled 18:2co6 was detected in fetal liver, brain and retina as 71%, 6%, and 21% of total label, respectively. In these tissues, 20-35% of the label was in long-chain u>6 fiitty acids (ω6-LCP) with the remaining 65-80% of label detected in the saturated and monounsaturated fatty acids (SFA/MFA) 16:0, 16:1, 18:0, and 18:1. These data show 1) 18:2ci>6 is converted to &>6-LCP over several days in the mother and is transferred to the fetus, and 2) recycling of 18:2ω6 into SFA/MFA is a dominant pathway in the fetus of the chow-fed orimate.
|Original language||English (US)|
|State||Published - Dec 1 1996|
ASJC Scopus subject areas
- Molecular Biology