Linkage mapping and physical localization of the major histocompatibility complex region of the marsupial Monodelphis domestica

N. Gouin, J. E. Deakin, K. B. Miska, R. D. Miller, C. M. Kammerer, J. A M Graves, J. L. VandeBerg, P. B. Samollow

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Abstract

We used genetic linkage mapping and fluorescence in situ hybridization (FISH) to conduct the first analysis of genic organization and chromosome localization of the major histocompatibility complex (MHC) of a marsupial, the gray, short-tailed opossum Monodelphis domestica. Family based linkage analyses of two M. domestica MHC Class I genes (UA1, UG) and three MHC Class II genes (DAB, DMA, and DMB) revealed that these genes were tightly linked and positioned in the central region of linkage group 3 (LG3). This cluster of MHC genes was physically mapped to the centromeric region of chromosome 2q by FISH using a BAC clone containing the UA1 gene. An interesting finding from the linkage analyses is that sex-specific recombination rates were virtually identical within the MHC region. This stands in stark contrast to the genome-wide situation, wherein males exhibit approximately twice as much recombination as females, and could have evolutionary implications for maintaining equality between males and females in the ability to generate haplotype diversity in this region. These analyses also showed that three non-MHC genes that flank the MHC region on human chromosome 6, myelin oligodendrocyte glycoprotein (MOG), bone morphogenetic protein 6 (BMP6), and prolactin (PRL), are split among two separate linkage groups (chromosomes) in M. domestica. Comparative analysis with eight other vertebrate species suggests strong conservation of the BMP6-PRL synteny among birds and mammals, although the BMP6-PRL-MHC-ME1 synteny is not conserved.

Original languageEnglish (US)
Pages (from-to)277-285
Number of pages9
JournalCytogenetic and Genome Research
Volume112
Issue number3-4
DOIs
StatePublished - 2006
Externally publishedYes

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Monodelphis
Marsupialia
Chromosome Mapping
Major Histocompatibility Complex
Bone Morphogenetic Protein 6
Prolactin
Synteny
Chromosomes
Fluorescence In Situ Hybridization
Genetic Recombination
Genes
Myelin-Oligodendrocyte Glycoprotein
MHC Class I Genes
MHC Class II Genes
Chromosomes, Human, Pair 6
Genetic Linkage
Human Chromosomes
Haplotypes
Birds
Vertebrates

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Gouin, N., Deakin, J. E., Miska, K. B., Miller, R. D., Kammerer, C. M., Graves, J. A. M., ... Samollow, P. B. (2006). Linkage mapping and physical localization of the major histocompatibility complex region of the marsupial Monodelphis domestica. Cytogenetic and Genome Research, 112(3-4), 277-285. https://doi.org/10.1159/000089882

Linkage mapping and physical localization of the major histocompatibility complex region of the marsupial Monodelphis domestica. / Gouin, N.; Deakin, J. E.; Miska, K. B.; Miller, R. D.; Kammerer, C. M.; Graves, J. A M; VandeBerg, J. L.; Samollow, P. B.

In: Cytogenetic and Genome Research, Vol. 112, No. 3-4, 2006, p. 277-285.

Research output: Contribution to journalArticle

Gouin, N, Deakin, JE, Miska, KB, Miller, RD, Kammerer, CM, Graves, JAM, VandeBerg, JL & Samollow, PB 2006, 'Linkage mapping and physical localization of the major histocompatibility complex region of the marsupial Monodelphis domestica', Cytogenetic and Genome Research, vol. 112, no. 3-4, pp. 277-285. https://doi.org/10.1159/000089882
Gouin, N. ; Deakin, J. E. ; Miska, K. B. ; Miller, R. D. ; Kammerer, C. M. ; Graves, J. A M ; VandeBerg, J. L. ; Samollow, P. B. / Linkage mapping and physical localization of the major histocompatibility complex region of the marsupial Monodelphis domestica. In: Cytogenetic and Genome Research. 2006 ; Vol. 112, No. 3-4. pp. 277-285.
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