Lineage tracing of primary human pancreatic acinar and ductal cells for studying acinar-to-ductal metaplasia

Jun Liu; Pei Wang

doi:10.1007/978-1-4939-8879-2_5

Lineage tracing of primary human pancreatic acinar and ductal cells for studying acinar-to-ductal metaplasia

Jun Liu
, Pei Wang

Department of Cell Systems & Anatomy

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Acinar-to-ductal metaplasia may play important roles in the development of various pancreatic diseases. Here, we describe a method to induce ADM in primary human cells in 3D culture. We developed a flow cytometry lineage tracing strategy to identify and sort viable acinar, ductal, and acinar-derived ductal-like cells for further molecular and functional analysis.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press
Pages	55-62
Number of pages	8
DOIs	https://doi.org/10.1007/978-1-4939-8879-2_5
State	Published - 2019

Publication series

Name	Methods in Molecular Biology
Volume	1882
ISSN (Print)	1064-3745

Keywords

3D culture
Acinar-to-ductal metaplasia
Flow cytometry
Human exocrine pancreatic cells
Lineage tracing

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-8879-2_5

Cite this

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abstract = "Acinar-to-ductal metaplasia may play important roles in the development of various pancreatic diseases. Here, we describe a method to induce ADM in primary human cells in 3D culture. We developed a flow cytometry lineage tracing strategy to identify and sort viable acinar, ductal, and acinar-derived ductal-like cells for further molecular and functional analysis.",

keywords = "3D culture, Acinar-to-ductal metaplasia, Flow cytometry, Human exocrine pancreatic cells, Lineage tracing",

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language = "English (US)",

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AU - Wang, Pei

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AB - Acinar-to-ductal metaplasia may play important roles in the development of various pancreatic diseases. Here, we describe a method to induce ADM in primary human cells in 3D culture. We developed a flow cytometry lineage tracing strategy to identify and sort viable acinar, ductal, and acinar-derived ductal-like cells for further molecular and functional analysis.

KW - 3D culture

KW - Acinar-to-ductal metaplasia

KW - Flow cytometry

KW - Human exocrine pancreatic cells

KW - Lineage tracing

UR - https://www.scopus.com/pages/publications/85055632898

UR - https://www.scopus.com/pages/publications/85055632898#tab=citedBy

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DO - 10.1007/978-1-4939-8879-2_5

M3 - Chapter

C2 - 30378043

AN - SCOPUS:85055632898

T3 - Methods in Molecular Biology

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EP - 62

BT - Methods in Molecular Biology

PB - Humana Press

ER -

Lineage tracing of primary human pancreatic acinar and ductal cells for studying acinar-to-ductal metaplasia

Abstract

Publication series

Keywords

ASJC Scopus subject areas

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