Lineage tracing of primary human pancreatic acinar and ductal cells for studying acinar-to-ductal metaplasia

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Acinar-to-ductal metaplasia may play important roles in the development of various pancreatic diseases. Here, we describe a method to induce ADM in primary human cells in 3D culture. We developed a flow cytometry lineage tracing strategy to identify and sort viable acinar, ductal, and acinar-derived ductal-like cells for further molecular and functional analysis.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages55-62
Number of pages8
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1882
ISSN (Print)1064-3745

Keywords

  • 3D culture
  • Acinar-to-ductal metaplasia
  • Flow cytometry
  • Human exocrine pancreatic cells
  • Lineage tracing

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Lineage tracing of primary human pancreatic acinar and ductal cells for studying acinar-to-ductal metaplasia'. Together they form a unique fingerprint.

  • Cite this