Lineage tracing of primary human pancreatic acinar and ductal cells for studying acinar-to-ductal metaplasia

Jun Liu, Pei Wang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Acinar-to-ductal metaplasia may play important roles in the development of various pancreatic diseases. Here, we describe a method to induce ADM in primary human cells in 3D culture. We developed a flow cytometry lineage tracing strategy to identify and sort viable acinar, ductal, and acinar-derived ductal-like cells for further molecular and functional analysis.

LanguageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages55-62
Number of pages8
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1882
ISSN (Print)1064-3745

Fingerprint

Acinar Cells
Metaplasia
Pancreatic Diseases
Flow Cytometry

Keywords

  • 3D culture
  • Acinar-to-ductal metaplasia
  • Flow cytometry
  • Human exocrine pancreatic cells
  • Lineage tracing

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Liu, J., & Wang, P. (2019). Lineage tracing of primary human pancreatic acinar and ductal cells for studying acinar-to-ductal metaplasia. In Methods in Molecular Biology (pp. 55-62). (Methods in Molecular Biology; Vol. 1882). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8879-2_5

Lineage tracing of primary human pancreatic acinar and ductal cells for studying acinar-to-ductal metaplasia. / Liu, Jun; Wang, Pei.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 55-62 (Methods in Molecular Biology; Vol. 1882).

Research output: Chapter in Book/Report/Conference proceedingChapter

Liu, J & Wang, P 2019, Lineage tracing of primary human pancreatic acinar and ductal cells for studying acinar-to-ductal metaplasia. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1882, Humana Press Inc., pp. 55-62. https://doi.org/10.1007/978-1-4939-8879-2_5
Liu J, Wang P. Lineage tracing of primary human pancreatic acinar and ductal cells for studying acinar-to-ductal metaplasia. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 55-62. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8879-2_5
Liu, Jun ; Wang, Pei. / Lineage tracing of primary human pancreatic acinar and ductal cells for studying acinar-to-ductal metaplasia. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 55-62 (Methods in Molecular Biology).
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