Ligand binding and structural changes associated with allostery in yeast NAD +-specific isocitrate dehydrogenase

Lee McAlister-Henn

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Yeast NAD +-specific isocitrate dehydrogenase (IDH) is an octameric enzyme composed of four each of regulatory IDH1 and catalytic IDH2 subunits that share 42% sequence identity. IDH2 contains catalytic isocitrate/Mg 2+ and NAD + binding sites whereas IDH1 contains homologous binding sites, respectively, for cooperative binding of isocitrate and for allosteric binding of AMP. Ligand binding is highly ordered in vitro, and IDH exhibits the unusual property of half-site binding for all ligands. The structures of IDH solved in the absence or presence of ligands have shown: (a) a heterodimer to be the basic structural/functional unit of the enzyme, (b) the organization of heterodimers to form tetramer and octamer structures, (c) structural differences that may underlie cooperative and allosteric regulatory mechanisms, and (d) the possibility for formation of a disulfide bond that could reduce catalytic activity. In vivo analyses of mutant enzymes have elucidated the physiological importance of catalytic activity and allosteric regulation of this tricarboxylic acid cycle enzyme. Other studies have established the importance of a disulfide bond in regulation of IDH activity in vivo, as well as contributions of this bond to the property of half-site ligand binding exhibited by the wild-type enzyme.

Original languageEnglish (US)
Pages (from-to)112-117
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume519
Issue number2
DOIs
StatePublished - Mar 15 2012

Fingerprint

Isocitrate Dehydrogenase
NAD
Yeast
Yeasts
Ligands
Binding Sites
Enzymes
Disulfides
Catalyst activity
Allosteric Regulation
Citric Acid Cycle
Adenosine Monophosphate
Catalytic Domain

Keywords

  • Allosteric regulation
  • Disulfide bond
  • Half-site ligand binding
  • Isocitrate dehydrogenase
  • Tricarboxylic acid cycle

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Ligand binding and structural changes associated with allostery in yeast NAD +-specific isocitrate dehydrogenase. / McAlister-Henn, Lee.

In: Archives of Biochemistry and Biophysics, Vol. 519, No. 2, 15.03.2012, p. 112-117.

Research output: Contribution to journalArticle

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