TY - JOUR
T1 - Levels of membrane fluidity in the spinal cord and the brain in an animal model of amyotrophic lateral sclerosis
AU - Miana-Mena, Francisco Javier
AU - Piedrafita, Eduardo
AU - González-Mingot, Cristina
AU - Larrodé, Pilar
AU - Muñoz, María Jesús
AU - Martínez-Ballarín, Enrique
AU - Reiter, Russel J.
AU - Osta, Rosario
AU - García, Joaquín J.
N1 - Funding Information:
Acknowledgements This work was supported by grants from the “Universidad de Zaragoza” (UZ2007-BIO-11); the “Gobierno de Aragón” (Aging and Oxidative Stress Physiology, Grant No. B40); the “Instituto de Estudios Altoaragoneses”; and funds from the “Fondo de Investigación Sanitaria” of Spain (PI071133). The authors have no conflicts of interest to declare.
PY - 2011/4
Y1 - 2011/4
N2 - A mutant form of the copper/zinc superoxide dismutase (SOD1) protein is found in some patients with amyotrophic lateral sclerosis (ALS). Alteration of the activity of this antioxidant enzyme leads to an oxidative stress imbalance, which damages the structure of lipids and proteins in the CNS. Using fluorescence spectroscopy, we monitored membrane fluidity in the spinal cord and the brain in a widely used animal model of ALS, the SODG93A mouse, which develops symptoms similar to ALS with an accelerated course. Our results show that the membrane fluidity of the spinal cord in this animal model significantly decreased in symptomatic animals compared with age-matched littermate controls. To the best of our knowledge, this is the first report showing that membrane fluidity is affected in the spinal cord of a SOD G93A animal model of ALS. Changes in membrane fluidity likely contribute substantially to alterations in cell membrane functions in the nervous tissue from SODG93A mice.
AB - A mutant form of the copper/zinc superoxide dismutase (SOD1) protein is found in some patients with amyotrophic lateral sclerosis (ALS). Alteration of the activity of this antioxidant enzyme leads to an oxidative stress imbalance, which damages the structure of lipids and proteins in the CNS. Using fluorescence spectroscopy, we monitored membrane fluidity in the spinal cord and the brain in a widely used animal model of ALS, the SODG93A mouse, which develops symptoms similar to ALS with an accelerated course. Our results show that the membrane fluidity of the spinal cord in this animal model significantly decreased in symptomatic animals compared with age-matched littermate controls. To the best of our knowledge, this is the first report showing that membrane fluidity is affected in the spinal cord of a SOD G93A animal model of ALS. Changes in membrane fluidity likely contribute substantially to alterations in cell membrane functions in the nervous tissue from SODG93A mice.
KW - Amyotrophic lateral sclerosis
KW - Brain
KW - G93A
KW - Membrane fluidity
KW - Oxidative stress
KW - SOD
KW - Spinal cord
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U2 - 10.1007/s10863-011-9348-5
DO - 10.1007/s10863-011-9348-5
M3 - Article
C2 - 21451979
AN - SCOPUS:79958253398
SN - 0145-479X
VL - 43
SP - 181
EP - 186
JO - Journal of Bioenergetics and Biomembranes
JF - Journal of Bioenergetics and Biomembranes
IS - 2
ER -