In a patient with leukemic reticuloendotheliosis (LRE) purified LRE cells could be obtained for the investigation of their structural and functional characteristics. The cells bore surface immunoglobulin and had complement receptors but did not bear F(c) receptors and did not form rosettes with sheep erythrocytes. By electron microscopy, the cells were observed to contain typical ribosome lamella structures and to phagocytize both 0.81 μm latex particles and complement-coated zymosan particles. They were adherent to both glass and nylon wool fibers. The mitogenic response to erythroagglutinating phytohemagglutinin was normal in magnitude but delayed chronologically. The binding of 125I-labeled plant lectins was used to chatacterize the surface topography of LRE cells. The results of these studies indicated that the LRE cell surface differed significantly from the surface of normal T and B lymphocytes and chronic lymphatic leukemia cells. The LRE cells were capable of both stimulating and responding in a one-way mixed lymphocyte culture. However, the LRE cells were not active as effector cells of either cell-mediated lympholysis, a T cell function, or antibody-dependent cellular cytotoxicity, a null cell function. In contrast, they were effector cells of lectin-induced cellular cytotoxicity showing that they did possess the capacity to function as cytotoxic effector cells. These data indicated that the LRE cell in this patient had surface and functional characteristics of both lymphocytes and monocytes.
|Original language||English (US)|
|Number of pages||13|
|State||Published - 1977|
ASJC Scopus subject areas
- Cell Biology