@inbook{e2a087d617b340d98da41c033c113c77,
title = "Lentiviral infection of mouse bone marrow cells for hematopoietic stem cell transplantation",
abstract = "Lentiviral vectors are increasingly used as efficient gene transfer tools in the experimental and clinical gene therapy treatment of acquired and inherited genetic diseases. Hematopoietic stem cells (HSCs) are characterized by the capacity for self-renewal, as well as multi-lineage differentiation and maintenance of the lymphohematopoietic system throughout life. As such, HSC transplantation (HSCT) has proven to be a powerful therapeutic modality for the treatment of both malignant and nonmalignant disorders. Transduction of lentiviral vectors into HSCs may offer long-term stable expression of a therapeutic gene in both preclinical and clinical settings. The purpose of this chapter is to describe an optimized procedure for lentiviral transduction of mouse HSCs followed by HSCT.",
keywords = "Bone marrow cells, Co-transfection, Hematopoietic stem cell transplantation, Lentiviral vector, Viral transduction",
author = "Cang Chen and Guderyon, {Michael J.} and Guo Ge and Clark, {Robert A.} and Senlin Li",
note = "Funding Information: Working with lentiviral vectors must follow the Centers for Disease Control and Prevention (CDC) guidelines for “Biosafety in Microbiological and Biomedical Laboratories” and adhere to the National Institutes of Health (NIH) requirements of “Biosafety Considerations for Research with Lentiviral Vectors.” The following safety equipment must be used when working with lentiviruses, (1) certified class II biological safety cabinets, (2) sealed centrifuge rotors and tubes, and (3) vacuum lines equipped with in-line HEPA filters. The following personal protective equipment (PPE) must be worn when working with lentiviruses, double gloves, lab coats, and eye protection. Funding Information: The establishment of this method was supported by a Merit Review grant (2I01BX000737) from the Department of Veterans Affairs Biomedical Laboratory Research & Development Program and by the William and Ella Owens Medical Research Foundation.",
year = "2019",
doi = "10.1007/978-1-4939-9007-8_16",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "205--213",
booktitle = "Methods in Molecular Biology",
address = "United States",
}