Lentiviral Infection of Mouse Bone Marrow Cells for Hematopoietic Stem Cell Transplantation

Cang Chen; Michael J. Guderyon; Guo Ge; Robert A Clark; Senlin Li

doi:10.1007/978-1-4939-9007-8_16

Lentiviral Infection of Mouse Bone Marrow Cells for Hematopoietic Stem Cell Transplantation

Cang Chen, Michael J. Guderyon, Guo Ge, Robert A Clark, Senlin Li

Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Lentiviral vectors are increasingly used as efficient gene transfer tools in the experimental and clinical gene therapy treatment of acquired and inherited genetic diseases. Hematopoietic stem cells (HSCs) are characterized by the capacity for self-renewal, as well as multi-lineage differentiation and maintenance of the lymphohematopoietic system throughout life. As such, HSC transplantation (HSCT) has proven to be a powerful therapeutic modality for the treatment of both malignant and nonmalignant disorders. Transduction of lentiviral vectors into HSCs may offer long-term stable expression of a therapeutic gene in both preclinical and clinical settings. The purpose of this chapter is to describe an optimized procedure for lentiviral transduction of mouse HSCs followed by HSCT.

Original language	English (US)
Pages (from-to)	205-213
Number of pages	9
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	1919
DOIs	https://doi.org/10.1007/978-1-4939-9007-8_16
State	Published - Jan 1 2019

Keywords

Bone marrow cells
Co-transfection
Hematopoietic stem cell transplantation
Lentiviral vector
Viral transduction

ASJC Scopus subject areas

Molecular Biology
Genetics

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