Abstract
Lentiviral vectors are increasingly used as efficient gene transfer tools in the experimental and clinical gene therapy treatment of acquired and inherited genetic diseases. Hematopoietic stem cells (HSCs) are characterized by the capacity for self-renewal, as well as multi-lineage differentiation and maintenance of the lymphohematopoietic system throughout life. As such, HSC transplantation (HSCT) has proven to be a powerful therapeutic modality for the treatment of both malignant and nonmalignant disorders. Transduction of lentiviral vectors into HSCs may offer long-term stable expression of a therapeutic gene in both preclinical and clinical settings. The purpose of this chapter is to describe an optimized procedure for lentiviral transduction of mouse HSCs followed by HSCT.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 205-213 |
| Number of pages | 9 |
| Journal | Methods in molecular biology (Clifton, N.J.) |
| Volume | 1919 |
| DOIs | |
| State | Published - Jan 1 2019 |
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Keywords
- Bone marrow cells
- Co-transfection
- Hematopoietic stem cell transplantation
- Lentiviral vector
- Viral transduction
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Lentiviral Infection of Mouse Bone Marrow Cells for Hematopoietic Stem Cell Transplantation. / Chen, Cang; Guderyon, Michael J.; Ge, Guo; Clark, Robert A; Li, Senlin.
In: Methods in molecular biology (Clifton, N.J.), Vol. 1919, 01.01.2019, p. 205-213.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lentiviral Infection of Mouse Bone Marrow Cells for Hematopoietic Stem Cell Transplantation
AU - Chen, Cang
AU - Guderyon, Michael J.
AU - Ge, Guo
AU - Clark, Robert A
AU - Li, Senlin
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Lentiviral vectors are increasingly used as efficient gene transfer tools in the experimental and clinical gene therapy treatment of acquired and inherited genetic diseases. Hematopoietic stem cells (HSCs) are characterized by the capacity for self-renewal, as well as multi-lineage differentiation and maintenance of the lymphohematopoietic system throughout life. As such, HSC transplantation (HSCT) has proven to be a powerful therapeutic modality for the treatment of both malignant and nonmalignant disorders. Transduction of lentiviral vectors into HSCs may offer long-term stable expression of a therapeutic gene in both preclinical and clinical settings. The purpose of this chapter is to describe an optimized procedure for lentiviral transduction of mouse HSCs followed by HSCT.
AB - Lentiviral vectors are increasingly used as efficient gene transfer tools in the experimental and clinical gene therapy treatment of acquired and inherited genetic diseases. Hematopoietic stem cells (HSCs) are characterized by the capacity for self-renewal, as well as multi-lineage differentiation and maintenance of the lymphohematopoietic system throughout life. As such, HSC transplantation (HSCT) has proven to be a powerful therapeutic modality for the treatment of both malignant and nonmalignant disorders. Transduction of lentiviral vectors into HSCs may offer long-term stable expression of a therapeutic gene in both preclinical and clinical settings. The purpose of this chapter is to describe an optimized procedure for lentiviral transduction of mouse HSCs followed by HSCT.
KW - Bone marrow cells
KW - Co-transfection
KW - Hematopoietic stem cell transplantation
KW - Lentiviral vector
KW - Viral transduction
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UR - http://www.scopus.com/inward/citedby.url?scp=85060168306&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-9007-8_16
DO - 10.1007/978-1-4939-9007-8_16
M3 - Article
C2 - 30656632
AN - SCOPUS:85060168306
VL - 1919
SP - 205
EP - 213
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
SN - 1064-3745
ER -