Laser speckle imaging of rat retinal blood flow with hybrid temporal and spatial analysis method

Haiying Cheng, Yumei Yan, Timothy Q. Duong

Research output: Contribution to journalConference articlepeer-review

6 Scopus citations

Abstract

Noninvasive monitoring of blood flow in retinal circulation will reveal the progression and treatment of ocular disorders, such as diabetic retinopathy, age-related macular degeneration and glaucoma. A non-invasive and direct BF measurement technique with high spatial-temporal resolution is needed for retinal imaging. Laser speckle imaging (LSI) is such a method. Currently, there are two analysis methods for LSI: spatial statistics LSI (SS-LSI) and temporal statistical LSI (TS-LSI). Comparing these two analysis methods, SS-LSI has higher signal to noise ratio (SNR) and TS- LSI is less susceptible to artifacts from stationary speckle. We proposed a hybrid temporal and spatial analysis method (HTS-LSI) to measure the retinal blood flow. Gas challenge experiment was performed and images were analyzed by HTS-LSI. Results showed that HTS-LSI can not only remove the stationary speckle but also increase the SNR. Under 100% O2, retinal BF decreased by 20-30%. This was consistent with the results observed with laser Doppler technique. As retinal blood flow is a critical physiological parameter and its perturbation has been implicated in the early stages of many retinal diseases, HTS-LSI will be an efficient method in early detection of retina diseases.

Original languageEnglish (US)
Article number716304
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7163
DOIs
StatePublished - May 13 2009
EventOphthalmic Technologies XIX - San Jose, CA, United States
Duration: Jan 24 2009Jan 26 2009

Keywords

  • Hybrid
  • Laser speckle imaging
  • Retinal blood flow
  • Signal to noise ratio
  • Spatial statistical
  • stationary speckle
  • Temporal statistical

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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