Laser speckle contrast imaging of blood flow in rat retinas using an endoscope

Adrien Ponticorvo, Damon Cardenas, Andrew K. Dunn, Daniel Ts'o, Timothy Q. Duong

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Laser speckle contrast imaging (LSCI) offers a costeffective means to image blood flow in vivo. However, it is not commonly used to image rodent retinas because of the challenges associated with imaging through the curved cornea and delivering light through the highly scattering lens. A solution to overcome these problems by using LSCI in conjunction with an endoscope to obtain high spatiotemporal blood flow images is described. Its utility is demonstrated by imaging blood flow changes in rat retinas using hyperoxic, hypercapnic, and visual (flicker) stimulations. Hypercapnia increases blood flow, hyperoxia decreases blood flow, and visual stimulation increases blood flow in the retina relative to basal conditions. The time-to-peak of the LSCI response to visual stimulation is also measured. This approach may prove useful to investigate dysregulation in blood flow-evoked responses in retinal diseases and to evaluate treatment strategies in rodents.

Original languageEnglish (US)
Article number090501
JournalJournal of biomedical optics
Volume18
Issue number9
DOIs
StatePublished - 2013

Keywords

  • Laser speckle imaging
  • flicker stimulation
  • gas challenge
  • retinal imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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