Laser fluorescence microscopy of nuclear protein targeting by synthetic signal peptides

H. Fan, R. Peters, R. E. Lanford

Research output: Contribution to journalConference articlepeer-review

Abstract

Immunoglobulin G was conjugated with synthetic peptides containing the nuclear localization sequence of the simian virus 40 T-antigen. The peptide-protein conjugates were injected into the cytoplasm of hepatoma cells. Nucleocytoplasmic transport of the conjugates was studied by both immunofluorescence staining of fixed cells and laser microfluorimetry of living cells. The results obtained by the two methods were compared and discussed with regard to previous studies of nucleocytoplasmic protein transport.

Original languageEnglish (US)
Pages (from-to)402-405
Number of pages4
JournalBerichte der Bunsengesellschaft/Physical Chemistry Chemical Physics
Volume93
Issue number3
DOIs
StatePublished - 1989
Externally publishedYes
EventDiscussion Meeting of The Deutsche Bunsen-Gesellschaft fuer Physikalische Chemie - Heildelberg, West Ger
Duration: Aug 29 1988Sep 1 1988

ASJC Scopus subject areas

  • Chemical Engineering(all)

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