Laser fluorescence microscopy of nuclear protein targeting by synthetic signal peptides

H. Fan, R. Peters, R. E. Lanford

    Research output: Contribution to journalConference article

    Abstract

    Immunoglobulin G was conjugated with synthetic peptides containing the nuclear localization sequence of the simian virus 40 T-antigen. The peptide-protein conjugates were injected into the cytoplasm of hepatoma cells. Nucleocytoplasmic transport of the conjugates was studied by both immunofluorescence staining of fixed cells and laser microfluorimetry of living cells. The results obtained by the two methods were compared and discussed with regard to previous studies of nucleocytoplasmic protein transport.

    Original languageEnglish (US)
    Pages (from-to)402-405
    Number of pages4
    JournalBerichte der Bunsengesellschaft/Physical Chemistry Chemical Physics
    Volume93
    Issue number3
    DOIs
    StatePublished - Jan 1 1989
    EventDiscussion Meeting of The Deutsche Bunsen-Gesellschaft fuer Physikalische Chemie - Heildelberg, West Ger
    Duration: Aug 29 1988Sep 1 1988

    ASJC Scopus subject areas

    • Chemical Engineering(all)

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