This chapter will describe the technique known as laser Doppler fl owmetry and its use in animals. Because the technique is based on light, it permits noninvasive measurements of perfusion in a tissue that can be illuminated, but perhaps its greatest strength is that it provides a continuous measurement. However, the technique also has signifi cant weaknesses, and it is technically challenging to use properly. On balance, it has added signifi cantly to our knowledge of ocular blood fl ow and will likely continue to be a valuable tool to increase our understanding of the physiology and pathophysiology of the eye.
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