Abstract
Emerging technologies allow broad profiling of the cancer genome for differential DNA methylation relative to benign cells. Herein, bisulfite-modified DNA from lymph nodes with either reactive hyperplasia or follicular lymphoma (FL) were analyzed using a commercial C/UpG genotyping assay. Two hundred fifty-nine differentially methylated targets (DMT) distributed among 183 unique genes were identified in FL. Comparison of matched formalin-fixed, paraffin-embedded and frozen surgical pathology replicates showed the complete preservation of the cancer methylome among differently archived tissue specimens. Analysis of the DMT profile is consistent with a pervasive epigenomic remodeling process in FLthat affects predominantly nonlymphoid genes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 758-764 |
| Number of pages | 7 |
| Journal | Cancer Research |
| Volume | 69 |
| Issue number | 3 |
| DOIs | |
| State | Published - Feb 1 2009 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Large-scale profiling of archival lymph nodes reveals pervasive remodeling of the follicular lymphoma methylome. / Killian, J. Keith; Bilke, Sven; Davis, Sean; Walker, Robert L.; Killian, M. Scott; Jaeger, Erich B.; Chen, Yidong; Hipp, Jason; Pittaluga, Stefania; Raffeld, Mark; Cornelison, Robert; Smith, William I.; Bibikova, Marina; Fan, Jian Bing; Emmert-Buck, Michael R.; Jaffe, Elaine S.; Meltzer, Paul S.
In: Cancer Research, Vol. 69, No. 3, 01.02.2009, p. 758-764.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Large-scale profiling of archival lymph nodes reveals pervasive remodeling of the follicular lymphoma methylome
AU - Killian, J. Keith
AU - Bilke, Sven
AU - Davis, Sean
AU - Walker, Robert L.
AU - Killian, M. Scott
AU - Jaeger, Erich B.
AU - Chen, Yidong
AU - Hipp, Jason
AU - Pittaluga, Stefania
AU - Raffeld, Mark
AU - Cornelison, Robert
AU - Smith, William I.
AU - Bibikova, Marina
AU - Fan, Jian Bing
AU - Emmert-Buck, Michael R.
AU - Jaffe, Elaine S.
AU - Meltzer, Paul S.
PY - 2009/2/1
Y1 - 2009/2/1
N2 - Emerging technologies allow broad profiling of the cancer genome for differential DNA methylation relative to benign cells. Herein, bisulfite-modified DNA from lymph nodes with either reactive hyperplasia or follicular lymphoma (FL) were analyzed using a commercial C/UpG genotyping assay. Two hundred fifty-nine differentially methylated targets (DMT) distributed among 183 unique genes were identified in FL. Comparison of matched formalin-fixed, paraffin-embedded and frozen surgical pathology replicates showed the complete preservation of the cancer methylome among differently archived tissue specimens. Analysis of the DMT profile is consistent with a pervasive epigenomic remodeling process in FLthat affects predominantly nonlymphoid genes.
AB - Emerging technologies allow broad profiling of the cancer genome for differential DNA methylation relative to benign cells. Herein, bisulfite-modified DNA from lymph nodes with either reactive hyperplasia or follicular lymphoma (FL) were analyzed using a commercial C/UpG genotyping assay. Two hundred fifty-nine differentially methylated targets (DMT) distributed among 183 unique genes were identified in FL. Comparison of matched formalin-fixed, paraffin-embedded and frozen surgical pathology replicates showed the complete preservation of the cancer methylome among differently archived tissue specimens. Analysis of the DMT profile is consistent with a pervasive epigenomic remodeling process in FLthat affects predominantly nonlymphoid genes.
UR - http://www.scopus.com/inward/record.url?scp=59149098762&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=59149098762&partnerID=8YFLogxK
U2 - 10.1158/0008-5472.CAN-08-2984
DO - 10.1158/0008-5472.CAN-08-2984
M3 - Article
C2 - 19155300
AN - SCOPUS:59149098762
VL - 69
SP - 758
EP - 764
JO - Cancer Research
JF - Cancer Research
SN - 0008-5472
IS - 3
ER -