Large-scale profiling of archival lymph nodes reveals pervasive remodeling of the follicular lymphoma methylome

J. Keith Killian, Sven Bilke, Sean Davis, Robert L. Walker, M. Scott Killian, Erich B. Jaeger, Yidong Chen, Jason Hipp, Stefania Pittaluga, Mark Raffeld, Robert Cornelison, William I. Smith, Marina Bibikova, Jian Bing Fan, Michael R. Emmert-Buck, Elaine S. Jaffe, Paul S. Meltzer

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Emerging technologies allow broad profiling of the cancer genome for differential DNA methylation relative to benign cells. Herein, bisulfite-modified DNA from lymph nodes with either reactive hyperplasia or follicular lymphoma (FL) were analyzed using a commercial C/UpG genotyping assay. Two hundred fifty-nine differentially methylated targets (DMT) distributed among 183 unique genes were identified in FL. Comparison of matched formalin-fixed, paraffin-embedded and frozen surgical pathology replicates showed the complete preservation of the cancer methylome among differently archived tissue specimens. Analysis of the DMT profile is consistent with a pervasive epigenomic remodeling process in FLthat affects predominantly nonlymphoid genes.

Original languageEnglish (US)
Pages (from-to)758-764
Number of pages7
JournalCancer Research
Volume69
Issue number3
DOIs
StatePublished - Feb 1 2009
Externally publishedYes

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Follicular Lymphoma
Lymph Nodes
Surgical Pathology
DNA Methylation
Epigenomics
Paraffin
Formaldehyde
Genes
Hyperplasia
Neoplasms
Genome
Technology
DNA
hydrogen sulfite

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Killian, J. K., Bilke, S., Davis, S., Walker, R. L., Killian, M. S., Jaeger, E. B., ... Meltzer, P. S. (2009). Large-scale profiling of archival lymph nodes reveals pervasive remodeling of the follicular lymphoma methylome. Cancer Research, 69(3), 758-764. https://doi.org/10.1158/0008-5472.CAN-08-2984

Large-scale profiling of archival lymph nodes reveals pervasive remodeling of the follicular lymphoma methylome. / Killian, J. Keith; Bilke, Sven; Davis, Sean; Walker, Robert L.; Killian, M. Scott; Jaeger, Erich B.; Chen, Yidong; Hipp, Jason; Pittaluga, Stefania; Raffeld, Mark; Cornelison, Robert; Smith, William I.; Bibikova, Marina; Fan, Jian Bing; Emmert-Buck, Michael R.; Jaffe, Elaine S.; Meltzer, Paul S.

In: Cancer Research, Vol. 69, No. 3, 01.02.2009, p. 758-764.

Research output: Contribution to journalArticle

Killian, JK, Bilke, S, Davis, S, Walker, RL, Killian, MS, Jaeger, EB, Chen, Y, Hipp, J, Pittaluga, S, Raffeld, M, Cornelison, R, Smith, WI, Bibikova, M, Fan, JB, Emmert-Buck, MR, Jaffe, ES & Meltzer, PS 2009, 'Large-scale profiling of archival lymph nodes reveals pervasive remodeling of the follicular lymphoma methylome', Cancer Research, vol. 69, no. 3, pp. 758-764. https://doi.org/10.1158/0008-5472.CAN-08-2984
Killian, J. Keith ; Bilke, Sven ; Davis, Sean ; Walker, Robert L. ; Killian, M. Scott ; Jaeger, Erich B. ; Chen, Yidong ; Hipp, Jason ; Pittaluga, Stefania ; Raffeld, Mark ; Cornelison, Robert ; Smith, William I. ; Bibikova, Marina ; Fan, Jian Bing ; Emmert-Buck, Michael R. ; Jaffe, Elaine S. ; Meltzer, Paul S. / Large-scale profiling of archival lymph nodes reveals pervasive remodeling of the follicular lymphoma methylome. In: Cancer Research. 2009 ; Vol. 69, No. 3. pp. 758-764.
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