@article{f48e838458b24bbbb9341a5f5414d293,
title = "Lactate Elicits ER-Mitochondrial Mg2+ Dynamics to Integrate Cellular Metabolism",
abstract = "Lactate operates as a signaling molecule to control Mg2+ handling between the ER and mitochondria.",
keywords = "Mrs2, calcium, cancer, channel, endoplasmic reticulum, inflammation, lactate, magnesium, metabolism, mitochondria",
author = "Daw, {Cassidy C.} and Karthik Ramachandran and Enslow, {Benjamin T.} and Soumya Maity and Brian Bursic and Novello, {Matthew J.} and Rubannelsonkumar, {Cherubina S.} and Mashal, {Ayah H.} and Joel Ravichandran and Bakewell, {Terry M.} and Weiwei Wang and Kang Li and Madaris, {Travis R.} and Shannon, {Christopher E.} and Luke Norton and Soundarya Kandala and Jeffrey Caplan and Subramanya Srikantan and Stathopulos, {Peter B.} and Reeves, {W. Brian} and Muniswamy Madesh",
note = "Funding Information: We thank Maarten Merkx for sharing the pCMV-MagFRET-1, Jean Ross for TEM sample preparation and imaging, and Dr. Vernon Anderson for his constant support and encouragement to the M.M. lab. This research was funded by the National Institutes of Health (R01GM109882, R01HL086699, R01HL142673, R01GM135760, and 1S10RR027327) to M.M. This work was partly supported by DOD/DHP-CDMRP PR181598P-1 and San Antonio Partnership for Precision Therapeutics (SAPPT) to M.M. and NSERC-07171 to P.B.S. B.T.E. is supported by the NIH (TL1 TR002647 and K12 GM111726). C.C.D. K.R. B.T.E, S.M. C.S.R. A.H.M. J.R. S.S. S.K. and M.M. performed and analyzed experiments involving metabolite screen, imaging, biochemical assays, mouse genotyping and qRT-PCR, FRET analysis, mitochondrial bioenergetics, and molecular/cellular techniques. J.C. performed TEM imaging and analysis. C.C.D. T.M.B. C.S. and L.N. performed murine hepatocyte isolation. B.B. M.J.N. and P.B.S. designed and performed the recombinant protein work. C.C.D. K.R. W.W. K.L. K.R. B.T.E. S.M. T.R.M. W.B.R. S.S. S.K. and M.M. performed in vivo studies and data interpretation. K.R. B.T.E. S.M. W.B.R. P.B.S. S.S. and M.M. analyzed and interpreted experimental data. B.T.E. S.S. and M.M. wrote the manuscript. M.M. conceived and designed the study. All authors commented on the manuscript. All data needed to evaluate the conclusions in the paper are present in the paper or the Supplemental Information. M.M. is an inventor on a patent provisionally filed by UTHSA on lactate as a ligand for Mg2+ dynamics in physiology and disease. All other authors have no financial interests to declare. Funding Information: We thank Maarten Merkx for sharing the pCMV-MagFRET-1, Jean Ross for TEM sample preparation and imaging, and Dr. Vernon Anderson for his constant support and encouragement to the M.M. lab. This research was funded by the National Institutes of Health ( R01GM109882 , R01HL086699 , R01HL142673 , R01GM135760 , and 1S10RR027327 ) to M.M. This work was partly supported by DOD/DHP-CDMRP PR181598P-1 and San Antonio Partnership for Precision Therapeutics (SAPPT) to M.M. and NSERC-07171 to P.B.S. B.T.E. is supported by the NIH ( TL1 TR002647 and K12 GM111726 ). ",
year = "2020",
month = oct,
day = "15",
doi = "10.1016/j.cell.2020.08.049",
language = "English (US)",
volume = "183",
pages = "474--489.e17",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "2",
}