TY - JOUR
T1 - Lack of Involvement of 6-Hydroxymethylation in Benzo[a]pyrene Skin Tumor Initiation in Mice
AU - Slaga, T. J.
AU - Bracken, W. M.
AU - Viaje, A.
AU - Berry, D. L.
AU - Fischer, S. M.
AU - Miller, D. R.
N1 - Funding Information:
1 Received October 26, 1977; accepted March 22, 1978. 2 Supported jointly by Public Health Service (PHS) grant CA20076 from the National Cancer Institute (NCI) and by the U.S. Department of Energy under contract with Union Carbide Corporation. , Biology Division, Oak Ridge National Laboratory, P.O. Box Y, Oak Ridge, Tenn. 37830. 4 The University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences, Oak Ridge, Tenn. 37830. 5 Predoctoral fellow supported by PHS grant CA09J04 from NCI.
PY - 1978/8
Y1 - 1978/8
N2 - The skin tumor-initiating activities of benzo[a]py- rene (BP), 6-hydroxymethylbenzo[a]pyrene (6-OH-CH2-BP), and 6-methylbenzo[a]pyrene (6-CH3-BP), as well as the effects of 7, 8- benzoflavone (7, 8-BF), quercetin, and 1-benzylimidazole on their activity, were determined in outbred female CD(r)-1 mice by use of a two-stage system of tumorigenesis. The skin tumor- initiating activity of 6-OH-CH2-BP and 6-CH3-BP was 12.5 and 20%, respectively, of the activity of BP. 7, 8-BF had little effect on the skin tumor-initiating activity of 6-OH-CH2-BP and 6-CH3- BP. However, a dose-dependent inhibition of BP tumorigenesis by 7, 8-BF was noted. Quercetin and 1-benzylimidazole also inhibited BP skin tumor-initiating activity. These findings indicated that direct hydroxyméthylation of BP is not an important pathway in the activation of BP in mouse skin tumor initiation.
AB - The skin tumor-initiating activities of benzo[a]py- rene (BP), 6-hydroxymethylbenzo[a]pyrene (6-OH-CH2-BP), and 6-methylbenzo[a]pyrene (6-CH3-BP), as well as the effects of 7, 8- benzoflavone (7, 8-BF), quercetin, and 1-benzylimidazole on their activity, were determined in outbred female CD(r)-1 mice by use of a two-stage system of tumorigenesis. The skin tumor- initiating activity of 6-OH-CH2-BP and 6-CH3-BP was 12.5 and 20%, respectively, of the activity of BP. 7, 8-BF had little effect on the skin tumor-initiating activity of 6-OH-CH2-BP and 6-CH3- BP. However, a dose-dependent inhibition of BP tumorigenesis by 7, 8-BF was noted. Quercetin and 1-benzylimidazole also inhibited BP skin tumor-initiating activity. These findings indicated that direct hydroxyméthylation of BP is not an important pathway in the activation of BP in mouse skin tumor initiation.
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U2 - 10.1093/jnci/61.2.451
DO - 10.1093/jnci/61.2.451
M3 - Article
C2 - 277730
AN - SCOPUS:0018143079
SN - 0027-8874
VL - 61
SP - 451
EP - 455
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 2
ER -