The skin tumor-initiating activities of benzo[a]py- rene (BP), 6-hydroxymethylbenzo[a]pyrene (6-OH-CH2-BP), and 6-methylbenzo[a]pyrene (6-CH3-BP), as well as the effects of 7, 8- benzoflavone (7, 8-BF), quercetin, and 1-benzylimidazole on their activity, were determined in outbred female CD(r)-1 mice by use of a two-stage system of tumorigenesis. The skin tumor- initiating activity of 6-OH-CH2-BP and 6-CH3-BP was 12.5 and 20%, respectively, of the activity of BP. 7, 8-BF had little effect on the skin tumor-initiating activity of 6-OH-CH2-BP and 6-CH3- BP. However, a dose-dependent inhibition of BP tumorigenesis by 7, 8-BF was noted. Quercetin and 1-benzylimidazole also inhibited BP skin tumor-initiating activity. These findings indicated that direct hydroxyméthylation of BP is not an important pathway in the activation of BP in mouse skin tumor initiation.
ASJC Scopus subject areas
- Cancer Research