Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 8079-8083 |
| Number of pages | 5 |
| Journal | Journal of Virology |
| Volume | 69 |
| Issue number | 12 |
| State | Published - Dec 1995 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
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Lack of detection of negative-strand hepatitis C virus RNA in peripheral blood mononuclear cells and other extrahepatic tissues by the highly strand-specific rTth reverse transcriptase PCR. / Lanford, Robert E.; Chavez, Deborah; Chisari, Francis V.; Sureau, Camille.
In: Journal of Virology, Vol. 69, No. 12, 12.1995, p. 8079-8083.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Lack of detection of negative-strand hepatitis C virus RNA in peripheral blood mononuclear cells and other extrahepatic tissues by the highly strand-specific rTth reverse transcriptase PCR
AU - Lanford, Robert E.
AU - Chavez, Deborah
AU - Chisari, Francis V.
AU - Sureau, Camille
PY - 1995/12
Y1 - 1995/12
N2 - To further explore the controversial potential for extrahepatic replication of hepatitis C virus (HCV), the highly strand-specific rTth method of reverse transcriptase PCR was used to examine sera, liver, peripheral blood mononuclear cells, and other extrahepatic tissues from HCV-infected chimpanzees and humans. Positive-strand HCV RNA was present in the liver at approximately 10-fold-higher levels than negative-strand HCV RNA. No negative-strand RNA was detected in peripheral blood mononuclear cells or other extrahepatic tissues despite the presence of abundant positive-strand RNA. These data demonstrate that within the limits of sensitivity of this highly strand-specific reverse transcriptase PCR method, no extrahepatic replication of HCV was detected.
AB - To further explore the controversial potential for extrahepatic replication of hepatitis C virus (HCV), the highly strand-specific rTth method of reverse transcriptase PCR was used to examine sera, liver, peripheral blood mononuclear cells, and other extrahepatic tissues from HCV-infected chimpanzees and humans. Positive-strand HCV RNA was present in the liver at approximately 10-fold-higher levels than negative-strand HCV RNA. No negative-strand RNA was detected in peripheral blood mononuclear cells or other extrahepatic tissues despite the presence of abundant positive-strand RNA. These data demonstrate that within the limits of sensitivity of this highly strand-specific reverse transcriptase PCR method, no extrahepatic replication of HCV was detected.
UR - http://www.scopus.com/inward/record.url?scp=0028818269&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028818269&partnerID=8YFLogxK
M3 - Article
C2 - 7494326
AN - SCOPUS:0028818269
VL - 69
SP - 8079
EP - 8083
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 12
ER -