Lack of detection of negative-strand hepatitis C virus RNA in peripheral blood mononuclear cells and other extrahepatic tissues by the highly strand-specific rTth reverse transcriptase PCR

Robert E. Lanford, Deborah Chavez, Francis V. Chisari, Camille Sureau

Research output: Contribution to journalArticle

226 Citations (Scopus)

Abstract

Original languageEnglish
Pages (from-to)8079-8083
Number of pages5
JournalJournal of Virology
Volume69
Issue number12
StatePublished - Dec 1995
Externally publishedYes

Fingerprint

Hepatitis C virus
mononuclear leukocytes
Reverse Transcriptase Polymerase Chain Reaction
Hepacivirus
Blood Cells
reverse transcriptase polymerase chain reaction
RNA
liver
Pan troglodytes
Liver
tissues
Serum
methodology

ASJC Scopus subject areas

  • Immunology

Cite this

Lack of detection of negative-strand hepatitis C virus RNA in peripheral blood mononuclear cells and other extrahepatic tissues by the highly strand-specific rTth reverse transcriptase PCR. / Lanford, Robert E.; Chavez, Deborah; Chisari, Francis V.; Sureau, Camille.

In: Journal of Virology, Vol. 69, No. 12, 12.1995, p. 8079-8083.

Research output: Contribution to journalArticle

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abstract = "To further explore the controversial potential for extrahepatic replication of hepatitis C virus (HCV), the highly strand-specific rTth method of reverse transcriptase PCR was used to examine sera, liver, peripheral blood mononuclear cells, and other extrahepatic tissues from HCV-infected chimpanzees and humans. Positive-strand HCV RNA was present in the liver at approximately 10-fold-higher levels than negative-strand HCV RNA. No negative-strand RNA was detected in peripheral blood mononuclear cells or other extrahepatic tissues despite the presence of abundant positive-strand RNA. These data demonstrate that within the limits of sensitivity of this highly strand-specific reverse transcriptase PCR method, no extrahepatic replication of HCV was detected.",
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AU - Sureau, Camille

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AB - To further explore the controversial potential for extrahepatic replication of hepatitis C virus (HCV), the highly strand-specific rTth method of reverse transcriptase PCR was used to examine sera, liver, peripheral blood mononuclear cells, and other extrahepatic tissues from HCV-infected chimpanzees and humans. Positive-strand HCV RNA was present in the liver at approximately 10-fold-higher levels than negative-strand HCV RNA. No negative-strand RNA was detected in peripheral blood mononuclear cells or other extrahepatic tissues despite the presence of abundant positive-strand RNA. These data demonstrate that within the limits of sensitivity of this highly strand-specific reverse transcriptase PCR method, no extrahepatic replication of HCV was detected.

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