Lack of detection of negative-strand hepatitis C virus RNA in peripheral blood mononuclear cells and other extrahepatic tissues by the highly strand- specific rTth reverse transcriptase PCR

R. E. Lanford, D. Chavez, F. V. Chisari, C. Sureau

    Research output: Contribution to journalArticlepeer-review

    227 Scopus citations

    Abstract

    To further explore the controversial potential for extrahepatic replication of hepatitis C virus (HCV), the highly strand-specific rTth method of reverse transcriptase PCR was used to examine sera, liver, peripheral blood mononuclear cells, and other extrahepatic tissues from HCV- infected chimpanzees and humans. Positive-strand HCV RNA was present in the liver at approximately 10-fold-higher levels than negative-strand HCV RNA. No negative-strand RNA was detected in peripheral blood mononuclear cells or other extrahepatic tissues despite the presence of abundant positive-strand RNA. These data demonstrate that within the limits of sensitivity of this highly strand-specific reverse transcriptase PCR method, no extrahepatic replication of HCV was detected.

    Original languageEnglish (US)
    Pages (from-to)8079-8083
    Number of pages5
    JournalJournal of virology
    Volume69
    Issue number12
    DOIs
    StatePublished - 1995

    ASJC Scopus subject areas

    • Microbiology
    • Immunology
    • Insect Science
    • Virology

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