Laboratory cultivation of caenorhabditis elegans and other free-living nematodes

Lan M. Caldicott, Pamela L Larsen, Donald L. Riddle

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

This chapter describes the cultivation of caenorhabditis elegans and other free-living nematodes in the laboratory. An adult C. elegans is about 1.5 mm long and, under optimal laboratory conditions, has a life cycle of approximately 3 days. There are two sexes, males and self-fertile hermaphrodites that are readily distinguishable as adults. The animals are transparent throughout the life cycle, permitting the observation of cell divisions in living animals using a differential interference microscopy. Tools for manipulating individual nematodes on plates are made by anchoring a 1.5-cm piece of 32-gauge platinum wire in a 6 inch inoculating loop holder or in a Pasteur pipette. For best results the end of the wire used for manipulation should be flattened and, if necessary, rounded to remove sharp edges. Remove supernatant and re-suspend in 15 ml of M9 buffer, divide between two 15-ml tubes, and place on ice. When cold, add 7.5 ml of cold 60% sucrose to each tube. Mix by inversion and centrifuge immediately at 1500 RCF for 5 min.

Original languageEnglish (US)
Title of host publicationCell Biology, Four-Volume Set
PublisherElsevier Inc.
Pages157-162
Number of pages6
Volume1
ISBN (Print)9780121647308
DOIs
StatePublished - 2006

Fingerprint

Caenorhabditis elegans
Life Cycle Stages
Life cycle
Animals
Wire
Interference Microscopy
Centrifuges
Ice
Platinum
Cell Division
Gages
Sucrose
Microscopic examination
Buffers
Cells
Observation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Caldicott, L. M., Larsen, P. L., & Riddle, D. L. (2006). Laboratory cultivation of caenorhabditis elegans and other free-living nematodes. In Cell Biology, Four-Volume Set (Vol. 1, pp. 157-162). Elsevier Inc.. https://doi.org/10.1016/B978-012164730-8/50020-4

Laboratory cultivation of caenorhabditis elegans and other free-living nematodes. / Caldicott, Lan M.; Larsen, Pamela L; Riddle, Donald L.

Cell Biology, Four-Volume Set. Vol. 1 Elsevier Inc., 2006. p. 157-162.

Research output: Chapter in Book/Report/Conference proceedingChapter

Caldicott, LM, Larsen, PL & Riddle, DL 2006, Laboratory cultivation of caenorhabditis elegans and other free-living nematodes. in Cell Biology, Four-Volume Set. vol. 1, Elsevier Inc., pp. 157-162. https://doi.org/10.1016/B978-012164730-8/50020-4
Caldicott LM, Larsen PL, Riddle DL. Laboratory cultivation of caenorhabditis elegans and other free-living nematodes. In Cell Biology, Four-Volume Set. Vol. 1. Elsevier Inc. 2006. p. 157-162 https://doi.org/10.1016/B978-012164730-8/50020-4
Caldicott, Lan M. ; Larsen, Pamela L ; Riddle, Donald L. / Laboratory cultivation of caenorhabditis elegans and other free-living nematodes. Cell Biology, Four-Volume Set. Vol. 1 Elsevier Inc., 2006. pp. 157-162
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