La Crosse virus nonstructural protein NSs counteracts the effects of short interfering RNA

Samantha S. Soldan, Matthew L. Plassmeyer, Meghan K. Matukonis, Francisco González-Scarano

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Through a process known as RNA interference (RNAi), double-stranded short interfering RNAs (siRNAs) silence gene expression in a sequence-specific manner. Recently, several viral proteins, including the non-structural protein NSs of tomato spotted wilt virus (a plant-infecting bunyavirus), the interferon antagonist protein NS1 of influenza virus, and the E3L protein of vaccinia virus, have been shown to function as suppressors of RNAi, presumably as a counterdefense against cellular mechanisms that decrease viral production. La Crosse virus (LACV), a member of the California serogroup of orthobunyaviruses, has a trisegmented negative-stranded genome comprised of large (L), medium (M), and small (S) segments. To develop a strategy for segment-specific inhibition of transcription, we designed 13 synthetic siRNAs targeting specific RNA segments of the LACV genome that decreased LACV replication and antigen expression in mammalian (293T) and insect (C6/36) cells. Furthermore, NSs, a LACV nonstructural protein, markedly inhibited RNAi directed both against an LACV M segment construct and against a host gene (glyeraldehyde-3-phosphate dehydrogenase), suggesting a possible role for this viral protein in the suppression of RNA silencing. Segment-specific siRNAs will be useful as a tool to analyze LACV transcription and replication and to obtain recombinant viruses. Additionally, NSs will help us to identify molecular pathways involved in RNAi and further define its role in the innate immune system.

Original languageEnglish (US)
Pages (from-to)234-244
Number of pages11
JournalJournal of Virology
Volume79
Issue number1
DOIs
StatePublished - Jan 2005
Externally publishedYes

Fingerprint

La Crosse virus
California encephalitis virus
small interfering RNA
Small Interfering RNA
RNA Interference
RNA interference
Orthobunyavirus
Proteins
proteins
Virus Replication
Tospovirus
Viral Nonstructural Proteins
transcription (genetics)
Genome
Tomato spotted wilt virus
Vaccinia virus
genome
Double-Stranded RNA
viral proteins
double-stranded RNA

ASJC Scopus subject areas

  • Immunology

Cite this

Soldan, S. S., Plassmeyer, M. L., Matukonis, M. K., & González-Scarano, F. (2005). La Crosse virus nonstructural protein NSs counteracts the effects of short interfering RNA. Journal of Virology, 79(1), 234-244. https://doi.org/10.1128/JVI.79.1.234-244.2005

La Crosse virus nonstructural protein NSs counteracts the effects of short interfering RNA. / Soldan, Samantha S.; Plassmeyer, Matthew L.; Matukonis, Meghan K.; González-Scarano, Francisco.

In: Journal of Virology, Vol. 79, No. 1, 01.2005, p. 234-244.

Research output: Contribution to journalArticle

Soldan, SS, Plassmeyer, ML, Matukonis, MK & González-Scarano, F 2005, 'La Crosse virus nonstructural protein NSs counteracts the effects of short interfering RNA', Journal of Virology, vol. 79, no. 1, pp. 234-244. https://doi.org/10.1128/JVI.79.1.234-244.2005
Soldan, Samantha S. ; Plassmeyer, Matthew L. ; Matukonis, Meghan K. ; González-Scarano, Francisco. / La Crosse virus nonstructural protein NSs counteracts the effects of short interfering RNA. In: Journal of Virology. 2005 ; Vol. 79, No. 1. pp. 234-244.
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