La Crosse virions contain a primer-stimulated RNA polymerase and a methylated cap-dependent endonuclease

J. L. Patterson, B. Holloway, D. Kolakofsky

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Purified La Crosse virions in vitro were found to transcribe their negative polarity (-) RNA genomes. This polymerase activity was stimulated by oligonucleotides such as (A)(n)G, cap analogs such as m7GpppAm, and natural mRNAs such as alfalfa mosaic virus RNA 4. For (A)(n)G- and alfalfa mosaic virus RNA 4-stimulated reactions, evidence is presented that these RNAs stimulate activity by acting as primers for viral transcription. The cap analogs appear to stimulate activity via an alternative mechanism. Purified La Crosse virions were also found to contain an endonuclease which specifically cleaves alfalfa mosaic virus RNA 4 when this RNA contains a methylated cap group.

Original languageEnglish (US)
Pages (from-to)215-222
Number of pages8
JournalJournal of Virology
Volume52
Issue number1
StatePublished - 1984
Externally publishedYes

Fingerprint

DNA-Directed RNA Polymerases
DNA-directed RNA polymerase
virion
Virion
Alfalfa mosaic virus
RNA
Endonucleases
oligonucleotides
Oligonucleotides
methylated cap-dependent endonuclease
transcription (genetics)
Genome
Messenger RNA
genome

ASJC Scopus subject areas

  • Immunology

Cite this

La Crosse virions contain a primer-stimulated RNA polymerase and a methylated cap-dependent endonuclease. / Patterson, J. L.; Holloway, B.; Kolakofsky, D.

In: Journal of Virology, Vol. 52, No. 1, 1984, p. 215-222.

Research output: Contribution to journalArticle

Patterson, JL, Holloway, B & Kolakofsky, D 1984, 'La Crosse virions contain a primer-stimulated RNA polymerase and a methylated cap-dependent endonuclease', Journal of Virology, vol. 52, no. 1, pp. 215-222.
Patterson, J. L. ; Holloway, B. ; Kolakofsky, D. / La Crosse virions contain a primer-stimulated RNA polymerase and a methylated cap-dependent endonuclease. In: Journal of Virology. 1984 ; Vol. 52, No. 1. pp. 215-222.
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