L-arginine restores the depressed cardiac output and regional perfusion after trauma-hemorrhage

M. K. Angele, N. Smail, P. Wang, W. G. Cioffi, K. I. Bland, I. H. Chaudry, T. R. Billiar, Basil A Pruitt, W. G. Cheadle, A. H. Harken

Research output: Contribution to journalArticle

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Abstract

Background. Previous studies indicate that vascular endothelial cell dysfunction occurs early after trauma-hemorrhage and may contribute to further alterations in tissue perfusion and cellular function. Because endothelial cell dysfunction is characterized by the reduced release of nitric oxide (NO) by endothelial constitutive NO synthase (cNOS), we tested hypothesis that administration of L-arginine (ie, the substrate for cNOS) after trauma and hemorrhage should have beneficial effects on depressed cardiac output and organ blood flow under those conditions. Methods. Rats underwent a laparotomy (ie, trauma induced) and were bled to and maintained at a mean arterial pressure of 40 mm Hg until 40% of maximal shed blood volume was returned in the form of Ringer's lactate solution. The animals were then resuscitated with 4 times the volume of the shed blood in the form of Ringer's lactate solution over 1 hour. 1-arginine (300 mg/kg body wt) or saline solution was infused intravenously during the first 15 minutes of resuscitation. Cardiac output and organ blood flow were determined by 85Sr- microspheres at 1.5 and 4 hours after the completion of resuscitation. Plasma interleukin-6 (IL-6) was determined by bioassay at 4 hours after resuscitation. Results. Cardiac output and blood flow in the kidneys, small intestine, and lungs decreased significantly after hemorrhage and resuscitation. In addition, portal blood flow and total hepatic perfusion were also significantly reduced. Administration of L-arginine at the onset of fluid resuscitation, however, restored the depressed cardiac output and tissue perfusion. Moreover, the up-regulated plasma levels of IL-6 were also attenuated by L-arginine administration. Conclusions. Because the adjuvant use of f-arginine restored the depressed cardiac output and organ blood flow and decreased plasma levels of IL-6, administration of this essential amino acid should be considered as a useful adjunct to fluid resuscitation for improving cardiovascular function in trauma victims.

Original languageEnglish (US)
Pages (from-to)394-402
Number of pages9
JournalSurgery
Volume124
Issue number2
DOIs
StatePublished - 1998
Externally publishedYes

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Resuscitation
Cardiac Output
Arginine
Perfusion
Hemorrhage
Wounds and Injuries
Interleukin-6
Blood Volume
Endothelial Cells
Essential Amino Acids
Nitric Oxide Synthase Type III
Microspheres
Sodium Chloride
Nitric Oxide Synthase
Biological Assay
Laparotomy
Small Intestine
Arterial Pressure
Nitric Oxide
Kidney

ASJC Scopus subject areas

  • Surgery

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Angele, M. K., Smail, N., Wang, P., Cioffi, W. G., Bland, K. I., Chaudry, I. H., ... Harken, A. H. (1998). L-arginine restores the depressed cardiac output and regional perfusion after trauma-hemorrhage. Surgery, 124(2), 394-402. https://doi.org/10.1016/S0039-6060(98)70146-1

L-arginine restores the depressed cardiac output and regional perfusion after trauma-hemorrhage. / Angele, M. K.; Smail, N.; Wang, P.; Cioffi, W. G.; Bland, K. I.; Chaudry, I. H.; Billiar, T. R.; Pruitt, Basil A; Cheadle, W. G.; Harken, A. H.

In: Surgery, Vol. 124, No. 2, 1998, p. 394-402.

Research output: Contribution to journalArticle

Angele, MK, Smail, N, Wang, P, Cioffi, WG, Bland, KI, Chaudry, IH, Billiar, TR, Pruitt, BA, Cheadle, WG & Harken, AH 1998, 'L-arginine restores the depressed cardiac output and regional perfusion after trauma-hemorrhage', Surgery, vol. 124, no. 2, pp. 394-402. https://doi.org/10.1016/S0039-6060(98)70146-1
Angele, M. K. ; Smail, N. ; Wang, P. ; Cioffi, W. G. ; Bland, K. I. ; Chaudry, I. H. ; Billiar, T. R. ; Pruitt, Basil A ; Cheadle, W. G. ; Harken, A. H. / L-arginine restores the depressed cardiac output and regional perfusion after trauma-hemorrhage. In: Surgery. 1998 ; Vol. 124, No. 2. pp. 394-402.
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abstract = "Background. Previous studies indicate that vascular endothelial cell dysfunction occurs early after trauma-hemorrhage and may contribute to further alterations in tissue perfusion and cellular function. Because endothelial cell dysfunction is characterized by the reduced release of nitric oxide (NO) by endothelial constitutive NO synthase (cNOS), we tested hypothesis that administration of L-arginine (ie, the substrate for cNOS) after trauma and hemorrhage should have beneficial effects on depressed cardiac output and organ blood flow under those conditions. Methods. Rats underwent a laparotomy (ie, trauma induced) and were bled to and maintained at a mean arterial pressure of 40 mm Hg until 40{\%} of maximal shed blood volume was returned in the form of Ringer's lactate solution. The animals were then resuscitated with 4 times the volume of the shed blood in the form of Ringer's lactate solution over 1 hour. 1-arginine (300 mg/kg body wt) or saline solution was infused intravenously during the first 15 minutes of resuscitation. Cardiac output and organ blood flow were determined by 85Sr- microspheres at 1.5 and 4 hours after the completion of resuscitation. Plasma interleukin-6 (IL-6) was determined by bioassay at 4 hours after resuscitation. Results. Cardiac output and blood flow in the kidneys, small intestine, and lungs decreased significantly after hemorrhage and resuscitation. In addition, portal blood flow and total hepatic perfusion were also significantly reduced. Administration of L-arginine at the onset of fluid resuscitation, however, restored the depressed cardiac output and tissue perfusion. Moreover, the up-regulated plasma levels of IL-6 were also attenuated by L-arginine administration. Conclusions. Because the adjuvant use of f-arginine restored the depressed cardiac output and organ blood flow and decreased plasma levels of IL-6, administration of this essential amino acid should be considered as a useful adjunct to fluid resuscitation for improving cardiovascular function in trauma victims.",
author = "Angele, {M. K.} and N. Smail and P. Wang and Cioffi, {W. G.} and Bland, {K. I.} and Chaudry, {I. H.} and Billiar, {T. R.} and Pruitt, {Basil A} and Cheadle, {W. G.} and Harken, {A. H.}",
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T1 - L-arginine restores the depressed cardiac output and regional perfusion after trauma-hemorrhage

AU - Angele, M. K.

AU - Smail, N.

AU - Wang, P.

AU - Cioffi, W. G.

AU - Bland, K. I.

AU - Chaudry, I. H.

AU - Billiar, T. R.

AU - Pruitt, Basil A

AU - Cheadle, W. G.

AU - Harken, A. H.

PY - 1998

Y1 - 1998

N2 - Background. Previous studies indicate that vascular endothelial cell dysfunction occurs early after trauma-hemorrhage and may contribute to further alterations in tissue perfusion and cellular function. Because endothelial cell dysfunction is characterized by the reduced release of nitric oxide (NO) by endothelial constitutive NO synthase (cNOS), we tested hypothesis that administration of L-arginine (ie, the substrate for cNOS) after trauma and hemorrhage should have beneficial effects on depressed cardiac output and organ blood flow under those conditions. Methods. Rats underwent a laparotomy (ie, trauma induced) and were bled to and maintained at a mean arterial pressure of 40 mm Hg until 40% of maximal shed blood volume was returned in the form of Ringer's lactate solution. The animals were then resuscitated with 4 times the volume of the shed blood in the form of Ringer's lactate solution over 1 hour. 1-arginine (300 mg/kg body wt) or saline solution was infused intravenously during the first 15 minutes of resuscitation. Cardiac output and organ blood flow were determined by 85Sr- microspheres at 1.5 and 4 hours after the completion of resuscitation. Plasma interleukin-6 (IL-6) was determined by bioassay at 4 hours after resuscitation. Results. Cardiac output and blood flow in the kidneys, small intestine, and lungs decreased significantly after hemorrhage and resuscitation. In addition, portal blood flow and total hepatic perfusion were also significantly reduced. Administration of L-arginine at the onset of fluid resuscitation, however, restored the depressed cardiac output and tissue perfusion. Moreover, the up-regulated plasma levels of IL-6 were also attenuated by L-arginine administration. Conclusions. Because the adjuvant use of f-arginine restored the depressed cardiac output and organ blood flow and decreased plasma levels of IL-6, administration of this essential amino acid should be considered as a useful adjunct to fluid resuscitation for improving cardiovascular function in trauma victims.

AB - Background. Previous studies indicate that vascular endothelial cell dysfunction occurs early after trauma-hemorrhage and may contribute to further alterations in tissue perfusion and cellular function. Because endothelial cell dysfunction is characterized by the reduced release of nitric oxide (NO) by endothelial constitutive NO synthase (cNOS), we tested hypothesis that administration of L-arginine (ie, the substrate for cNOS) after trauma and hemorrhage should have beneficial effects on depressed cardiac output and organ blood flow under those conditions. Methods. Rats underwent a laparotomy (ie, trauma induced) and were bled to and maintained at a mean arterial pressure of 40 mm Hg until 40% of maximal shed blood volume was returned in the form of Ringer's lactate solution. The animals were then resuscitated with 4 times the volume of the shed blood in the form of Ringer's lactate solution over 1 hour. 1-arginine (300 mg/kg body wt) or saline solution was infused intravenously during the first 15 minutes of resuscitation. Cardiac output and organ blood flow were determined by 85Sr- microspheres at 1.5 and 4 hours after the completion of resuscitation. Plasma interleukin-6 (IL-6) was determined by bioassay at 4 hours after resuscitation. Results. Cardiac output and blood flow in the kidneys, small intestine, and lungs decreased significantly after hemorrhage and resuscitation. In addition, portal blood flow and total hepatic perfusion were also significantly reduced. Administration of L-arginine at the onset of fluid resuscitation, however, restored the depressed cardiac output and tissue perfusion. Moreover, the up-regulated plasma levels of IL-6 were also attenuated by L-arginine administration. Conclusions. Because the adjuvant use of f-arginine restored the depressed cardiac output and organ blood flow and decreased plasma levels of IL-6, administration of this essential amino acid should be considered as a useful adjunct to fluid resuscitation for improving cardiovascular function in trauma victims.

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