K+-evoked dopamine release depends on a cytosolic Ca2+ pool regulated by N-type Ca2+ channels

Gabriel de Erausquin, Gary Brooker, Ingeborg Hanbauer

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Membrane depolarization evoked by 25-40 mM K+ elicited an immediate increase of somatic and neuritic [Ca2+]i in cultured dopaminergic neurons as measured by digital fluorescence microscope imaging. The rise of neuritic [Ca2+]i was inhibited by N-type but not L-type Ca2+ channel blockers, while the rise of somatic [Ca2+]i was prevented by both L- and N-type Ca2+ channel blockers. Similarly, depolarization-induced [3H]dopamine release was selectively attenuated by N-type Ca2+ channel blockers. The present results suggest that [3H]dopamine release from mesencephalic neuronal cell cultures relates to a Ca2+-dependent mechanism regulated by N-type channels located in the vicinity of the exocytotic sites within neuritic processes.

Original languageEnglish (US)
Pages (from-to)121-125
Number of pages5
JournalNeuroscience Letters
Volume145
Issue number2
DOIs
StatePublished - Oct 12 1992
Externally publishedYes

Keywords

  • Ca channel
  • Dopamine release
  • Fura-2 fluorescence
  • Mesencephalon
  • Primary culture
  • Single-cell calcium imaging
  • Spatial signal

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'K<sup>+</sup>-evoked dopamine release depends on a cytosolic Ca<sup>2+</sup> pool regulated by N-type Ca<sup>2+</sup> channels'. Together they form a unique fingerprint.

  • Cite this