Abstract
Membrane depolarization evoked by 25-40 mM K+ elicited an immediate increase of somatic and neuritic [Ca2+]i in cultured dopaminergic neurons as measured by digital fluorescence microscope imaging. The rise of neuritic [Ca2+]i was inhibited by N-type but not L-type Ca2+ channel blockers, while the rise of somatic [Ca2+]i was prevented by both L- and N-type Ca2+ channel blockers. Similarly, depolarization-induced [3H]dopamine release was selectively attenuated by N-type Ca2+ channel blockers. The present results suggest that [3H]dopamine release from mesencephalic neuronal cell cultures relates to a Ca2+-dependent mechanism regulated by N-type channels located in the vicinity of the exocytotic sites within neuritic processes.
Original language | English (US) |
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Pages (from-to) | 121-125 |
Number of pages | 5 |
Journal | Neuroscience Letters |
Volume | 145 |
Issue number | 2 |
DOIs | |
State | Published - Oct 12 1992 |
Externally published | Yes |
Keywords
- Ca channel
- Dopamine release
- Fura-2 fluorescence
- Mesencephalon
- Primary culture
- Single-cell calcium imaging
- Spatial signal
ASJC Scopus subject areas
- General Neuroscience