K+ channel currents in rat ventral prostate epithelial cells

Jun Hee Kim, Eun Kyung Hong, Hee Sook Choi, Seung Joon Oh, Kwang Myung Kim, Dae Yong Uhm, Sung Joon Kim

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

BACKGROUND. Electrophysiological function of the normal prostate has not been extensively studied. In particular, ion channel currents and their regulation have not been studied in freshly-isolated prostate cells. METHODS. Rat prostate secretory epithelial (RPSE) cells were isolated by collagenase treatment. Columnar epithelial cells were used for nystatin-perforated, whole-cell voltage clamp, and the intracellular Ca2+ concentration ([Ca2+]i) was measured using fura-2. RESULTS. Step-like depolarizing pulses (900 msec) starting from - 90 mV induced out-wardly rectifying K+ currents without inactivation. ACh (10 μM) or ATP (100 μM) increased the outward current and hyperpolarized the cell membrane potential. Ionomycin (0.1 μM), a Ca2+ ionophore, induced a similar increase in the outward current. TEA (5 mM), charybdotoxin (50 nM), and iberiotoxin (30 nM) inhibited the effect of ACh (or ATP) on the outward current, whereas apamin (100 nM) had no effect. The [Ca2+]i of RPSE cells was increased by ACh, ATP, and UTP. CONCLUSIONS. RPSE cells have iberiotoxin-sensitive Ca2+-activated K+ channels that may play an important role in the exocrine secretions of the prostate.

Original languageEnglish (US)
Pages (from-to)201-210
Number of pages10
JournalProstate
Volume51
Issue number3
DOIs
StatePublished - May 15 2002
Externally publishedYes

Keywords

  • Intracellular Ca
  • K channel
  • Muscarinic receptor
  • Prostate epithelium
  • Purinoceptor
  • Voltage clamp

ASJC Scopus subject areas

  • Oncology
  • Urology

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    Kim, J. H., Hong, E. K., Choi, H. S., Oh, S. J., Kim, K. M., Uhm, D. Y., & Kim, S. J. (2002). K+ channel currents in rat ventral prostate epithelial cells. Prostate, 51(3), 201-210. https://doi.org/10.1002/pros.10090