Kinetics of association and dissociation of colchicine-tubulin complex from brain and renal tubulin Evidence for the existence of multiple isotypes of tubulin in brain with differential affinity to colchicine

Asok Banerjee, Richard F. Luduena

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The kinetics of colchicine binding to bovine brain tubulin have been reported to be biphasic under pseudo first order conditions [(1978) Biochemistry 17, 4466-4472]. Unlike brain tubulin, the kinetics of colchicine binding to bovine renal tubulin are monophasic. The apparent on-rate constant for the binding of colchicine to renal tubulin is found to be very close to that of the faster binding component in brain tubulin. Similarly, the dissociation of colchicine-tubulin complex in the presence of iodide is biphasic for brain tubulin but monophasic for renal tubulin. Since brain and renal tubulin apparently differ in β-tubulin, our results suggest that the biphasic nature of the kinetics for bovine brain tubulin could possibly originate from the existence of multiple isotypes of tubulin differing in drug binding affinity.

Original languageEnglish (US)
Pages (from-to)103-107
Number of pages5
JournalFEBS Letters
Volume219
Issue number1
DOIs
StatePublished - Jul 13 1987

Fingerprint

Colchicine
Tubulin
Brain
Association reactions
Kidney
Kinetics
Biochemistry
Iodides
Rate constants

Keywords

  • (Brain, Kidney)
  • Biphasic association kinetics
  • Biphasic dissociation kinetics
  • Colchicine-tubulin
  • Fluorescence
  • Tubulin isotype

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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title = "Kinetics of association and dissociation of colchicine-tubulin complex from brain and renal tubulin Evidence for the existence of multiple isotypes of tubulin in brain with differential affinity to colchicine",
abstract = "The kinetics of colchicine binding to bovine brain tubulin have been reported to be biphasic under pseudo first order conditions [(1978) Biochemistry 17, 4466-4472]. Unlike brain tubulin, the kinetics of colchicine binding to bovine renal tubulin are monophasic. The apparent on-rate constant for the binding of colchicine to renal tubulin is found to be very close to that of the faster binding component in brain tubulin. Similarly, the dissociation of colchicine-tubulin complex in the presence of iodide is biphasic for brain tubulin but monophasic for renal tubulin. Since brain and renal tubulin apparently differ in β-tubulin, our results suggest that the biphasic nature of the kinetics for bovine brain tubulin could possibly originate from the existence of multiple isotypes of tubulin differing in drug binding affinity.",
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T1 - Kinetics of association and dissociation of colchicine-tubulin complex from brain and renal tubulin Evidence for the existence of multiple isotypes of tubulin in brain with differential affinity to colchicine

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N2 - The kinetics of colchicine binding to bovine brain tubulin have been reported to be biphasic under pseudo first order conditions [(1978) Biochemistry 17, 4466-4472]. Unlike brain tubulin, the kinetics of colchicine binding to bovine renal tubulin are monophasic. The apparent on-rate constant for the binding of colchicine to renal tubulin is found to be very close to that of the faster binding component in brain tubulin. Similarly, the dissociation of colchicine-tubulin complex in the presence of iodide is biphasic for brain tubulin but monophasic for renal tubulin. Since brain and renal tubulin apparently differ in β-tubulin, our results suggest that the biphasic nature of the kinetics for bovine brain tubulin could possibly originate from the existence of multiple isotypes of tubulin differing in drug binding affinity.

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