TY - JOUR
T1 - Kin17, a mouse nuclear zinc finger protein that binds preferentially to curved DNA
AU - Mazin, Alexander
AU - Timchenko, Tatiana
AU - Ménissier-de Murcia, Josiane
AU - Schreiber, Valérie
AU - Angulo, Jaime F.
AU - Gilbert De, Murcia
AU - Devoret, Raymond
N1 - Funding Information:
We thank M.Pierre for her help with the drawings and the references. We are grateful to A.Bailone and E.Bonnefoy and J.Rouviere-Yaniv for kindly providing strains and oligonucleotides. M.Saparbaev is thanked for his help in purifying the kin 17 proteins. We benefited from the advice of R.Benarous, M.Bianchi, C.Craescu, J-L.Danan, M.Dutreix, M.Marilley, B.Revet and M.Takahashi. A.Mazin benefited from fellowships of the International Agency for Research on Cancer (CIRC/R. 1938) and the Toxicological Society of the European Science Foundation. A.Mazin and T.Timchenko are grateful to the Association pour la Recherche sur le Cancer for post-doctoral fellowships and also to the Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, for granting them a leave of absence. This work would not have been possible without funds granted to R.Devoret from the EEC no. FI3PCT92-0028, the Association pour la Recherche sur le Cancer no. 6093 and the Fondation pour la Recherche Medicale.
PY - 1994/10/11
Y1 - 1994/10/11
N2 - Kin17 is a 45 kDa protein encoded by the KIN17 gene located on mouse chromosome 2, band A. The kin17 amino acid sequence predicts two domains, which were shown to be functional: (i) a bipartite nuclear localization signal (NLS) that can drive the protein to the cell nucleus, (ii) a bona fide zinc finger of the C2H2 type. The zinc finger is involved in kin17 binding to double-stranded DNA since a mutant deleted of the zinc finger, kin17δ1, showed reduced binding. Singlestranded DNA was bound poorly by kin17. Interestingly, we found that kin17 protein showed preferential binding to curved DNA from either pBR322 or synthetic oligonucleotides. Binding of kin17 to a non-curved DNA segment increased after we had inserted into it a short curved synthetic oligonucleotide. Kin17δ2, a mutant deleted of 110 amino acids at the C-terminal end, still exhibited preferential binding to curved DNA and so did kin17δ1, suggesting that a domain recognizing curved DNA is located in the protein core.
AB - Kin17 is a 45 kDa protein encoded by the KIN17 gene located on mouse chromosome 2, band A. The kin17 amino acid sequence predicts two domains, which were shown to be functional: (i) a bipartite nuclear localization signal (NLS) that can drive the protein to the cell nucleus, (ii) a bona fide zinc finger of the C2H2 type. The zinc finger is involved in kin17 binding to double-stranded DNA since a mutant deleted of the zinc finger, kin17δ1, showed reduced binding. Singlestranded DNA was bound poorly by kin17. Interestingly, we found that kin17 protein showed preferential binding to curved DNA from either pBR322 or synthetic oligonucleotides. Binding of kin17 to a non-curved DNA segment increased after we had inserted into it a short curved synthetic oligonucleotide. Kin17δ2, a mutant deleted of 110 amino acids at the C-terminal end, still exhibited preferential binding to curved DNA and so did kin17δ1, suggesting that a domain recognizing curved DNA is located in the protein core.
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U2 - 10.1093/nar/22.20.4335
DO - 10.1093/nar/22.20.4335
M3 - Article
C2 - 7937163
AN - SCOPUS:0027984291
SN - 0305-1048
VL - 22
SP - 4335
EP - 4341
JO - Nucleic acids research
JF - Nucleic acids research
IS - 20
ER -