Kin17, a mouse nuclear zinc finger protein that binds preferentially to curved DNA

Alexander Mazin, Tatiana Timchenko, Josiane Ménissier-de Murcia, Valérie Schreiber, Jaime F. Angulo, Murcia Gilbert De, Raymond Devoret

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Kin17 is a 45 kDa protein encoded by the KIN17 gene located on mouse chromosome 2, band A. The kin17 amino acid sequence predicts two domains, which were shown to be functional: (i) a bipartite nuclear localization signal (NLS) that can drive the protein to the cell nucleus, (ii) a bona fide zinc finger of the C2H2 type. The zinc finger is involved in kin17 binding to double-stranded DNA since a mutant deleted of the zinc finger, kin17δ1, showed reduced binding. Singlestranded DNA was bound poorly by kin17. Interestingly, we found that kin17 protein showed preferential binding to curved DNA from either pBR322 or synthetic oligonucleotides. Binding of kin17 to a non-curved DNA segment increased after we had inserted into it a short curved synthetic oligonucleotide. Kin17δ2, a mutant deleted of 110 amino acids at the C-terminal end, still exhibited preferential binding to curved DNA and so did kin17δ1, suggesting that a domain recognizing curved DNA is located in the protein core.

Original languageEnglish (US)
Pages (from-to)4335-4341
Number of pages7
JournalNucleic acids research
Volume22
Issue number20
DOIs
StatePublished - Oct 11 1994
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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