Key proteolytic cleavage site and full-length form of DSPP

Y. Sun, Y. Lu, Shuo Chen, M. Prasad, X. Wang, Q. Zhu, J. Zhang, H. Ball, J. Feng, W. T. Butler, C. Qin

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

It is known that dentin sialophosphoprotein (DSPP) is processed into NH2- and COOHterminal fragments, but its key cleavage site has not been identified, nor has its full-length form been discovered. The objectives of this study were to identify the key cleavage site during DSPP processing and to search for full-length DSPP in vivo. We generated a construct encoding DSPP, in which Asp452, a cleavage site residue, was replaced by Ala 452. The pulp-odontoblast complex and dentin were extracted, chromatographically separated, and assessed by Stains-All staining, Western immunoblotting, and mass spectrometry. These studies showed that the substitution of Asp452 by Ala452 completely blocks the cleavage of mouse DSPP in the transfected cells, indicating that the NH 2-terminal peptide bond of Asp452 is essential for the initiation of DSPP proteolytic processing. The results of this study revealed the presence of full-length DSPP and its processed fragments in extracts from the pulp/odontoblast and dentin.

Original languageEnglish (US)
Pages (from-to)498-503
Number of pages6
JournalJournal of Dental Research
Volume89
Issue number5
DOIs
StatePublished - May 2010

Fingerprint

Odontoblasts
Dentin
dentin sialophosphoprotein
Mass Spectrometry
Western Blotting
Staining and Labeling
Peptides
stains-all

Keywords

  • Dentin extracellular matrix
  • Dentin phosphoprotein
  • Dentin sialophosphoprotein
  • Dentin sialoprotein
  • Proteolytic processing

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Key proteolytic cleavage site and full-length form of DSPP. / Sun, Y.; Lu, Y.; Chen, Shuo; Prasad, M.; Wang, X.; Zhu, Q.; Zhang, J.; Ball, H.; Feng, J.; Butler, W. T.; Qin, C.

In: Journal of Dental Research, Vol. 89, No. 5, 05.2010, p. 498-503.

Research output: Contribution to journalArticle

Sun, Y, Lu, Y, Chen, S, Prasad, M, Wang, X, Zhu, Q, Zhang, J, Ball, H, Feng, J, Butler, WT & Qin, C 2010, 'Key proteolytic cleavage site and full-length form of DSPP', Journal of Dental Research, vol. 89, no. 5, pp. 498-503. https://doi.org/10.1177/0022034510363109
Sun, Y. ; Lu, Y. ; Chen, Shuo ; Prasad, M. ; Wang, X. ; Zhu, Q. ; Zhang, J. ; Ball, H. ; Feng, J. ; Butler, W. T. ; Qin, C. / Key proteolytic cleavage site and full-length form of DSPP. In: Journal of Dental Research. 2010 ; Vol. 89, No. 5. pp. 498-503.
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