TY - JOUR
T1 - Keratin protein expression during the development of conducting airway epithelium in nonhuman primates
AU - Huang, Tim H.
AU - George, Judith A.St
AU - Plopper, Charles G.
AU - Wu, Reen
N1 - Funding Information:
Acknowledgements. The authors wish to thank Chris Brown, Diane Cranz, and Alison Weir for thcir excellent technical assistance. Drs. T.T. Sun (New York University, School of Medicine, New York), D.R. Roop (NCI, Bethesda, Md), R.D. Cardiff (University of California, Davis, Calif.), and J.G. Rheinwald (Dana-Farber Cancer Institute, Harvard Medical School, Boston, Mass.) are thanked for the supply of keratin antibodies used in this study. We wish to thank Janet Perkins for editing the manuscript. This paper was supported in part by NIH Grants HL 28978 (to C.G.P.), HL 35635 (to R.W.), and a grant from the Cystic Fibrosis Foundation, G172 8-01 (to R.W.).
PY - 1989
Y1 - 1989
N2 - Keratin protein expression during the development of Rhesus monkey conducting airway epithelium was investigated by both biochemical and immunohistochemical methods. Keratin proteins were extracted from tracheal and intrapulmonary airway tissues of fetal (at 80- and 140-day gestational ages), neonatal, and adult animals. Using immunoblot analyses and immunohistochemistry with various monoclonal (AE1, AE3, AE8, 6.01 and 6.11) and monospecific antibodies (anti-50/55 and anti-40 kDa), the presence of keratins 5, 6, 8, 13, 14, and 19 [14] in adult airway epithelium were demonstrated. Except for keratin 13 (51 kDa), the remaining keratins could be immunologically detected in fetal and neonatal tissues. To further understand the nature of the synthesis of keratin 13 during development, airway epithelial cells from different ages were isolated and cultured in vitro. Cultured cells were labeled with 35S-methionine, and the patterns of keratin protein were analyzed by one- and two-dimensional sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Results indicated that the cultured airway cells synthesized additional keratins including 7,15,16,17,and 18. However, consistent with the in vivo finding, fetal cells synthesized less or no keratin 13. These in vivo and in vitro studies strongly suggest that the synthesis of the keratin 13 in monkey conducting airway epithelium is developmentally regulated.
AB - Keratin protein expression during the development of Rhesus monkey conducting airway epithelium was investigated by both biochemical and immunohistochemical methods. Keratin proteins were extracted from tracheal and intrapulmonary airway tissues of fetal (at 80- and 140-day gestational ages), neonatal, and adult animals. Using immunoblot analyses and immunohistochemistry with various monoclonal (AE1, AE3, AE8, 6.01 and 6.11) and monospecific antibodies (anti-50/55 and anti-40 kDa), the presence of keratins 5, 6, 8, 13, 14, and 19 [14] in adult airway epithelium were demonstrated. Except for keratin 13 (51 kDa), the remaining keratins could be immunologically detected in fetal and neonatal tissues. To further understand the nature of the synthesis of keratin 13 during development, airway epithelial cells from different ages were isolated and cultured in vitro. Cultured cells were labeled with 35S-methionine, and the patterns of keratin protein were analyzed by one- and two-dimensional sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Results indicated that the cultured airway cells synthesized additional keratins including 7,15,16,17,and 18. However, consistent with the in vivo finding, fetal cells synthesized less or no keratin 13. These in vivo and in vitro studies strongly suggest that the synthesis of the keratin 13 in monkey conducting airway epithelium is developmentally regulated.
UR - http://www.scopus.com/inward/record.url?scp=0024427571&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024427571&partnerID=8YFLogxK
U2 - 10.1111/j.1432-0436.1989.tb00735.x
DO - 10.1111/j.1432-0436.1989.tb00735.x
M3 - Article
C2 - 2478409
AN - SCOPUS:0024427571
SN - 0301-4681
VL - 41
SP - 78
EP - 86
JO - Differentiation
JF - Differentiation
IS - 1
ER -