Isotyping and quantitation of the humoral immune response to SARS-CoV-2

Krystal A. Goyins, Jieh Juen Yu, Sara B. Papp, Rachel Beddard, Ashlesh K. Murthy, James P. Chambers, Bernard P. Arulanandam

Research output: Contribution to journalArticlepeer-review

Abstract

Understanding the immune response to SARS-CoV-2 is important for development of effective diagnostics and vaccines. We report here a broad antibody response to SARS-CoV-2 spike protein receptor binding domain (RBD) in 100 convalescent patient plasma samples. Antibody isotypes IgA, IgM, and IgG exhibited significantly higher anti-RBD titers when compared to SARS-CoV-2 negative controls. IgG subtyping indicated IgG1 and IgG3 to be most abundant. Greater than 90 % of SARS-CoV-2 positive plasma samples tested exhibited significant neutralization capacity using a surrogate virus neutralization assay. Of the IgG subclasses, IgG1 and IgG3 exhibited the highest viral neutralization capacity; whereas, IgG2 and IgG4 viral neutralization was not observed. Comparison of SARS-CoV-2 elicited total IgG binding to emerging variant (alpha, beta, and delta) RBDs indicated decreased binding. Furthermore, neutralization by SARS-CoV-2 convalescent plasma of delta and omicron variant RBDs was significantly decreased suggesting that neutralizing antibodies in convalescent plasma are less effective in inhibiting variants currently in circulation.

Original languageEnglish (US)
Pages (from-to)1055-1060
Number of pages6
JournalExperimental Biology and Medicine
Volume247
Issue number12
DOIs
StatePublished - Jun 2022

Keywords

  • COVID-19
  • SARS-CoV-2
  • antibody
  • neutralization
  • plasma
  • variant

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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