Isotope dilution GC/MS determination of anandamide and other fatty acylethanolamides in rat blood plasma

Andrea Giuffrida, Daniele Piomelli

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Anandamide and allied fatty acylethanolamides (AEs) may act as signalling molecules in brain and peripheral tissues. In the present study, we describe an electron-impact gas chromatography/mass spectrometry (GC/MS) method based on isotope dilution, which may be used for the identification and quantification of anandamide and other AEs in biological matrices. The calibration curves for standard AEs were linear over the range 0-1,000 pmol (r2 = 0.99) with a coefficient of variation of 4% at 2.5 pmol. Detection and quantification limits were in the high fmol to low pmol range for all AEs. Using this method we measured nanomolar concentrations of three endogenous AEs in deproteinated rat blood plasma (anandamide: 5.2 pmol/ml; palmitylethanolamide: 16.7 pmol/ml; oleylethanolamide: 8.1 pmol/ml). These results are consistent with a regulatory role of anandamide and other AEs in peripheral tissues.

Original languageEnglish (US)
Pages (from-to)373-376
Number of pages4
JournalFEBS Letters
Volume422
Issue number3
DOIs
StatePublished - Feb 6 1998
Externally publishedYes

Fingerprint

Isotopes
Gas chromatography
Gas Chromatography-Mass Spectrometry
Dilution
Mass spectrometry
Rats
Blood
Plasmas
Tissue
Calibration
Limit of Detection
Brain
Electrons
Molecules
anandamide

Keywords

  • Anandamide
  • Cannabinoid
  • Fatty acylethanolamide
  • Gas chromatography
  • Mass spectrometry
  • Plasma

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Isotope dilution GC/MS determination of anandamide and other fatty acylethanolamides in rat blood plasma. / Giuffrida, Andrea; Piomelli, Daniele.

In: FEBS Letters, Vol. 422, No. 3, 06.02.1998, p. 373-376.

Research output: Contribution to journalArticle

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N2 - Anandamide and allied fatty acylethanolamides (AEs) may act as signalling molecules in brain and peripheral tissues. In the present study, we describe an electron-impact gas chromatography/mass spectrometry (GC/MS) method based on isotope dilution, which may be used for the identification and quantification of anandamide and other AEs in biological matrices. The calibration curves for standard AEs were linear over the range 0-1,000 pmol (r2 = 0.99) with a coefficient of variation of 4% at 2.5 pmol. Detection and quantification limits were in the high fmol to low pmol range for all AEs. Using this method we measured nanomolar concentrations of three endogenous AEs in deproteinated rat blood plasma (anandamide: 5.2 pmol/ml; palmitylethanolamide: 16.7 pmol/ml; oleylethanolamide: 8.1 pmol/ml). These results are consistent with a regulatory role of anandamide and other AEs in peripheral tissues.

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