Isopiestic determination of water binding by fish antifreeze glycoproteins

John G. Duman, Jean L. Patterson, John J. Kozak, Arthur L. DeVries

    Research output: Contribution to journalArticlepeer-review

    9 Scopus citations

    Abstract

    The effectiveness of water binding of antifreeze glycoproteins relative to hemoglobin, cytochrome c and polyvinylpyrrolidone was determined by analyzing results obtained in an isopiestic study at 25°C. The net weight of water which moved from a protein/NaCl aqueous sample to a saturated NaCl reference solution increased in the order: antifreeze glycoprotein, hemoglobin, polyvinylpyrrolidone and cytochrome c. Since the glycoproteins were least effective in transporting water we conclude that, of the proteins studied, the glycoprotein was most effective in binding water under equilibrium conditions at 25°C.

    Original languageEnglish (US)
    Pages (from-to)332-336
    Number of pages5
    JournalBBA - Protein Structure
    Volume626
    Issue number2
    DOIs
    StatePublished - Dec 16 1980

    Keywords

    • (Fish)
    • Antifreeze glycoprotein
    • Isopiestic study
    • Water binding

    ASJC Scopus subject areas

    • Medicine(all)

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