Isolation of type II epithelial cells from rabbit fetal lungs by adherence on an IgG-coated surface

A. Gupta, S. Kaushal, S. Majumdar, S. N. Sanyal

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The lung is comprised of about 40 different cell types, of which only 15% are type II cells. These are the major, if not the sole, source of synthesis and secretion of lung surfactant. To date a large number of methods have been described for the isolation of pure populations of type II cells using a wide variety of techniques, but most of these have employed differential centrifugation methods and have used adult rodents. The present study reports the isolation of type II cells from fetal rabbit lungs by the immunoglobin G plating method. Pure populations of fetal type II cells in high yield and with good viability were obtained by the procedure for the first time from rabbit fetal tissue.

Original languageEnglish (US)
Pages (from-to)799-802
Number of pages4
JournalExperientia
Volume52
Issue number8
StatePublished - 1996
Externally publishedYes

Fingerprint

Centrifugation
Plating
Surface-Active Agents
Immunoglobulin G
Epithelial Cells
Tissue
Rabbits
Lung
Cell Separation
Population
Rodentia
Fetus

Keywords

  • Adherence on IgG plates
  • Lung type II epithelial cell isolation
  • Pulmonary surfactant

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Gupta, A., Kaushal, S., Majumdar, S., & Sanyal, S. N. (1996). Isolation of type II epithelial cells from rabbit fetal lungs by adherence on an IgG-coated surface. Experientia, 52(8), 799-802.

Isolation of type II epithelial cells from rabbit fetal lungs by adherence on an IgG-coated surface. / Gupta, A.; Kaushal, S.; Majumdar, S.; Sanyal, S. N.

In: Experientia, Vol. 52, No. 8, 1996, p. 799-802.

Research output: Contribution to journalArticle

Gupta, A, Kaushal, S, Majumdar, S & Sanyal, SN 1996, 'Isolation of type II epithelial cells from rabbit fetal lungs by adherence on an IgG-coated surface', Experientia, vol. 52, no. 8, pp. 799-802.
Gupta, A. ; Kaushal, S. ; Majumdar, S. ; Sanyal, S. N. / Isolation of type II epithelial cells from rabbit fetal lungs by adherence on an IgG-coated surface. In: Experientia. 1996 ; Vol. 52, No. 8. pp. 799-802.
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