Isolation of Trypsins by Affinity Chromatography

Neal C. Robinson, Ross W. Tye, Hans Neurath, Kenneth A. Walsh

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Bovine α- and β-trypsin were separated by affinity chromatography on a column of chicken ovomucoid covalently bound to Sepharose. The two active fractions were selectively eluted by a pH gradient. The procedure was also applied to the purification of porcine and dogfish trypsins and to the isolation of trypsin from activated bovine pancreatic juice. Neither trypsinogen nor a-chymotrypsin was retarded by this column.

Original languageEnglish (US)
Pages (from-to)2743-2747
Number of pages5
Issue number14
StatePublished - Jul 1 1971

ASJC Scopus subject areas

  • Biochemistry


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