Isolation of Trypsins by Affinity Chromatography

Neal C. Robinson, Ross W. Tye, Hans Neurath, Kenneth A. Walsh

Research output: Contribution to journalArticle

107 Scopus citations

Abstract

Bovine α- and β-trypsin were separated by affinity chromatography on a column of chicken ovomucoid covalently bound to Sepharose. The two active fractions were selectively eluted by a pH gradient. The procedure was also applied to the purification of porcine and dogfish trypsins and to the isolation of trypsin from activated bovine pancreatic juice. Neither trypsinogen nor a-chymotrypsin was retarded by this column.

Original languageEnglish (US)
Pages (from-to)2743-2747
Number of pages5
JournalBiochemistry
Volume10
Issue number14
DOIs
StatePublished - Jul 1 1971

ASJC Scopus subject areas

  • Biochemistry

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  • Cite this

    Robinson, N. C., Tye, R. W., Neurath, H., & Walsh, K. A. (1971). Isolation of Trypsins by Affinity Chromatography. Biochemistry, 10(14), 2743-2747. https://doi.org/10.1021/bi00790a014