Isolation of the ends of La Crosse virus small RNA as a double-stranded structure

J. L. Patterson, D. Kolakofsky, B. P. Holloway, J. F. Obijeski

    Research output: Contribution to journalArticlepeer-review

    8 Scopus citations

    Abstract

    The genome of La Crosse virus, a member of the Bunyaviridae, is made up of three molecules. Circular nucleocapsid structures, in three size classes, have been isolated from La Crosse virus (Obijeski et al. J. Virol. 20:664-675, 1976). Recently, Obijeski et al. (Nucleic Acids Res. 8:2431-2438) have found that the 5' and 3' ends of each segment are complementary in sequence. We determined that a 5' and 3' end complementary structure, predicted by the rules of Tinoco et al. (Nature [London] 230:362-367), can and will anneal under certain conditions. This structure is resistant to RNase in high-salt medium and can be isolated in a reasonably high yield.

    Original languageEnglish (US)
    Pages (from-to)882-884
    Number of pages3
    JournalJournal of virology
    Volume45
    Issue number2
    DOIs
    StatePublished - 1983

    ASJC Scopus subject areas

    • Microbiology
    • Immunology
    • Insect Science
    • Virology

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