Isolation of Sertoli, Leydig, and spermatogenic cells from the mouse testis

Yao Fu Chang, Jennifer S. Lee-Chang, Subbarayalu Panneerdoss, James A. MacLean, Manjeet K. Rao

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

A thorough understanding of the events during mammalian spermatogenesis requires studying specific molecular signatures of individual testicular cell populations as well as their interaction in co-cultures. However, most purification techniques to isolate specific testicular cell populations are timeconsuming, require large numbers of animals, and/or are only able to isolate a few cell types. Here we describe a cost-effective and timesaving approach that uses a single protocol to enrich multiple testicular cell populations (Sertoli, Leydig, and several spermatogenic cell populations) from as few as one mouse. Our protocol combines rigorous enzymatic digestion of seminiferous tubules with counter-current centrifugal elutriation, yielding specific testicular cell populations with >80%?95% purity.

Original languageEnglish (US)
Pages (from-to)341-344
Number of pages4
JournalBioTechniques
Volume51
Issue number5
DOIs
StatePublished - Nov 2011

Keywords

  • Elutriation
  • Germ cell
  • Leydig cell
  • Sertoli cell
  • Spermatid
  • Spermatocyte
  • Spermatogenesis
  • Testis

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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