Abstract
A simple method for the Isolation of pure and highyield DNA from whole blood, suitable for restriction enzyme digestion, is described. The Steps of the procedure are as follows: Cell lysis with NH4C1, NaHCO3, EDTA; digestion with proteinase K in the presence of SDS; extraction with phenol-chloroform-isoamyl alcohol; and precipitation with ethanol. The 260 nm/280 nm absorbance ratio showed a mean value of 2, and the average yield of DNA was 212 µg/l. Such DNA preparations were found to be quite suitable for digestion by a variety of restriction endonucleases, as well as for the analysis of gene disorders by different biological methods. The method proposed appears to be useful in clinical chemistry laboratories.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 327-330 |
| Number of pages | 4 |
| Journal | Clinical Chemistry and Laboratory Medicine |
| Volume | 29 |
| Issue number | 5 |
| DOIs | |
| State | Published - 1991 |
| Externally published | Yes |
ASJC Scopus subject areas
- Clinical Biochemistry
- Biochemistry, medical