Isolation of Restrictible DNA

Elizabeta Topić, Jelica Gluhak

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

A simple method for the Isolation of pure and highyield DNA from whole blood, suitable for restriction enzyme digestion, is described. The Steps of the procedure are as follows: Cell lysis with NH4C1, NaHCO3, EDTA; digestion with proteinase K in the presence of SDS; extraction with phenol-chloroform-isoamyl alcohol; and precipitation with ethanol. The 260 nm/280 nm absorbance ratio showed a mean value of 2, and the average yield of DNA was 212 µg/l. Such DNA preparations were found to be quite suitable for digestion by a variety of restriction endonucleases, as well as for the analysis of gene disorders by different biological methods. The method proposed appears to be useful in clinical chemistry laboratories.

Original languageEnglish (US)
Pages (from-to)327-330
Number of pages4
JournalClinical Chemistry and Laboratory Medicine
Volume29
Issue number5
DOIs
StatePublished - 1991
Externally publishedYes

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ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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