Abstract
A simple method for the Isolation of pure and highyield DNA from whole blood, suitable for restriction enzyme digestion, is described. The Steps of the procedure are as follows: Cell lysis with NH4C1, NaHCO3, EDTA; digestion with proteinase K in the presence of SDS; extraction with phenol-chloroform-isoamyl alcohol; and precipitation with ethanol. The 260 nm/280 nm absorbance ratio showed a mean value of 2, and the average yield of DNA was 212 µg/l. Such DNA preparations were found to be quite suitable for digestion by a variety of restriction endonucleases, as well as for the analysis of gene disorders by different biological methods. The method proposed appears to be useful in clinical chemistry laboratories.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 327-330 |
| Number of pages | 4 |
| Journal | Clinical Chemistry and Laboratory Medicine |
| Volume | 29 |
| Issue number | 5 |
| DOIs | |
| State | Published - 1991 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Clinical Biochemistry
- Biochemistry, medical
Cite this
Isolation of Restrictible DNA. / Topić, Elizabeta; Gluhak, Jelica.
In: Clinical Chemistry and Laboratory Medicine, Vol. 29, No. 5, 1991, p. 327-330.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation of Restrictible DNA
AU - Topić, Elizabeta
AU - Gluhak, Jelica
PY - 1991
Y1 - 1991
N2 - A simple method for the Isolation of pure and highyield DNA from whole blood, suitable for restriction enzyme digestion, is described. The Steps of the procedure are as follows: Cell lysis with NH4C1, NaHCO3, EDTA; digestion with proteinase K in the presence of SDS; extraction with phenol-chloroform-isoamyl alcohol; and precipitation with ethanol. The 260 nm/280 nm absorbance ratio showed a mean value of 2, and the average yield of DNA was 212 µg/l. Such DNA preparations were found to be quite suitable for digestion by a variety of restriction endonucleases, as well as for the analysis of gene disorders by different biological methods. The method proposed appears to be useful in clinical chemistry laboratories.
AB - A simple method for the Isolation of pure and highyield DNA from whole blood, suitable for restriction enzyme digestion, is described. The Steps of the procedure are as follows: Cell lysis with NH4C1, NaHCO3, EDTA; digestion with proteinase K in the presence of SDS; extraction with phenol-chloroform-isoamyl alcohol; and precipitation with ethanol. The 260 nm/280 nm absorbance ratio showed a mean value of 2, and the average yield of DNA was 212 µg/l. Such DNA preparations were found to be quite suitable for digestion by a variety of restriction endonucleases, as well as for the analysis of gene disorders by different biological methods. The method proposed appears to be useful in clinical chemistry laboratories.
UR - http://www.scopus.com/inward/record.url?scp=84955845754&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84955845754&partnerID=8YFLogxK
U2 - 10.1515/cclm.1991.29.5.327
DO - 10.1515/cclm.1991.29.5.327
M3 - Article
AN - SCOPUS:84955845754
VL - 29
SP - 327
EP - 330
JO - Clinical Chemistry and Laboratory Medicine
JF - Clinical Chemistry and Laboratory Medicine
SN - 1434-6621
IS - 5
ER -