Isolation of restrictible DNA

E. Topic, J. Gluhak

Research output: Contribution to journalArticle

Abstract

A simple method for the isolation of pure and high-yield DNA from whole blood, suitable for restriction enzyme digestion, is described. The steps of the procedure are as follows: cell lysis with NH4Cl, NaHCO3, EDTA; digestion with proteinase K in the presence of SDS; extraction with phenol-chloroform-isoamyl alcohol; and precipitation with ethanol. The 260 nm/280 nm absorbance ratio showed a mean value of 2, and the average yield of DNA was 212 μg/l. Such DNA preparations were found to he quite suitable for digestion by a variety of restriction endonucleases, as well as for the analysis of gene disorders by different biological methods. The method proposed appears to be useful in clinical chemistry laboratories.

Original languageEnglish (US)
Pages (from-to)327-330
Number of pages4
JournalEuropean Journal of Clinical Chemistry and Clinical Biochemistry
Volume29
Issue number5
StatePublished - 1991
Externally publishedYes

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Digestion
DNA
Endopeptidase K
DNA Restriction Enzymes
Chloroform
Phenol
Edetic Acid
Clinical Chemistry
Blood
Ethanol
Genes
Enzymes
isopentyl alcohol

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Isolation of restrictible DNA. / Topic, E.; Gluhak, J.

In: European Journal of Clinical Chemistry and Clinical Biochemistry, Vol. 29, No. 5, 1991, p. 327-330.

Research output: Contribution to journalArticle

Topic, E. ; Gluhak, J. / Isolation of restrictible DNA. In: European Journal of Clinical Chemistry and Clinical Biochemistry. 1991 ; Vol. 29, No. 5. pp. 327-330.
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