A simple method for the isolation of pure and high-yield DNA from whole blood, suitable for restriction enzyme digestion, is described. The steps of the procedure are as follows: cell lysis with NH4Cl, NaHCO3, EDTA; digestion with proteinase K in the presence of SDS; extraction with phenol-chloroform-isoamyl alcohol; and precipitation with ethanol. The 260 nm/280 nm absorbance ratio showed a mean value of 2, and the average yield of DNA was 212 μg/l. Such DNA preparations were found to he quite suitable for digestion by a variety of restriction endonucleases, as well as for the analysis of gene disorders by different biological methods. The method proposed appears to be useful in clinical chemistry laboratories.
|Original language||English (US)|
|Number of pages||4|
|Journal||European Journal of Clinical Chemistry and Clinical Biochemistry|
|State||Published - 1991|
ASJC Scopus subject areas
- Clinical Biochemistry