Isolation of Coccidioides immitis F antigen by immunoaffinity chromatography with monospecific antiserum

R. A. Cox, L. A. Britt, R. A. Michael

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Detection of antibody to Coccidioides immitis F antigen is of proved value in the diagnosis of coccidioidomycosis. This antibody is demonstrable by use of an immunodiffusion assay with reference coccidioidin antigen and antiserum to C. immitis. Using a combination of lectin affinity and immunoaffinity chromatography, we isolated the F antigen from coccidioidin and prepared monospecific antibody to the purified antigen. The availability of these reagents will enable the development of a sensitive and specific assay for detecting serologic reactivity to this antigen.

Original languageEnglish (US)
Pages (from-to)227-232
Number of pages6
JournalInfection and immunity
Volume55
Issue number1
DOIs
StatePublished - 1987
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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