Isolation of a collagen-dependent cell attachment factor

Robert J. Klebe

Research output: Contribution to journalArticlepeer-review

397 Scopus citations


MOST untransformed mammalian cells require an appropriate surface for survival and growth in vitro; this phenomenon has been termed anchorage dependence1. Glass, tissue culture plastics, fibrin clots and collagen surfaces have long been recognised as substrates able to support the attachment and growth of cells. Since the cell plasma membrane is separated from plastics substrates by a 450 Å layer of electron opaque material 2, the nature of the 450 Å would appear to be more important in cell attachment than the chemical composition of the plastic. It is demonstrated here that cell attachment to collagen is mediated by a high molecular weight protein present in serum.

Original languageEnglish (US)
Pages (from-to)248-251
Number of pages4
Issue number5463
StatePublished - 1974
Externally publishedYes

ASJC Scopus subject areas

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