TY - JOUR
T1 - Isolation, characterization, and distribution of a 24-kDa proteoglycan in the urine of cachectic cancer and AIDS patients
AU - Figueroa, Julio E.
AU - Vijayagopal, Parakat
AU - Prasad, Anand
AU - Schapira, David V.
AU - Prasad, Chandan
N1 - Funding Information:
This research was supported in part by Catherine Vernice and Vincent Charles Vernice Research Fund in Endocrinology. The authors thank Ms. Anne Compliment for her superb and timely editorial assistance.
PY - 1999/1/27
Y1 - 1999/1/27
N2 - Substantial weight loss in individuals with AIDS or cancer is associated with a poor prognosis and increased mortality. We have isolated and partially characterized a proteoglycan (named azaftig) from the urine of a cancer patient experiencing weight loss. Furthermore, we have raised a polyclonal antibody to azaftig in rabbits and developed a procedure to measure the level of this proteoglycan in urine by Western blot. We report the presence of azaftig in the urine of cancer and AIDS patients experiencing weight loss, but not in the control or weight-stable subjects. The azaftig-like immunoreactivity was present in 69.2% (9/13) of patients with weight loss, but only in 27.0% (3/11) of weight-stable cancer or AIDS patients and none of the control subjects (n = 8).
AB - Substantial weight loss in individuals with AIDS or cancer is associated with a poor prognosis and increased mortality. We have isolated and partially characterized a proteoglycan (named azaftig) from the urine of a cancer patient experiencing weight loss. Furthermore, we have raised a polyclonal antibody to azaftig in rabbits and developed a procedure to measure the level of this proteoglycan in urine by Western blot. We report the presence of azaftig in the urine of cancer and AIDS patients experiencing weight loss, but not in the control or weight-stable subjects. The azaftig-like immunoreactivity was present in 69.2% (9/13) of patients with weight loss, but only in 27.0% (3/11) of weight-stable cancer or AIDS patients and none of the control subjects (n = 8).
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U2 - 10.1006/bbrc.1998.9985
DO - 10.1006/bbrc.1998.9985
M3 - Article
C2 - 9920793
AN - SCOPUS:0033608249
SN - 0006-291X
VL - 254
SP - 642
EP - 646
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -