Isolation and purification of self-renewable human neural stem cells for cell therapy in experimental model of ischemic stroke

Ricardo L. Azevedo-Pereira, Marcel M. Daadi

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Scopus citations

Abstract

Human embryonic stem cells (hESCs) are pluripotent with a strong self-renewable ability making them a virtually unlimited source of neural cells for structural repair in neurological disorders. Currently, hESCs are one of the most promising cell sources amenable for commercialization of off-shelf cell therapy products. However, along with this strong proliferative capacity of hESCs comes the tumorigenic potential of these cells after transplantation. Thus, the isolation and purification of a homogeneous, population of neural stem cells (hNSCs) are of paramount importance to avoid tumor formation in the host brain. This chapter describes the isolation, neuralization, and long-term perpetuation of hNSCs derived from hESCs through use of specific mitogenic growth factors and the preparation of hNSCs for transplantation in an experimental model of stroke. Additionally, we describe methods to analyze the stroke and size of grafts using magnetic resonance imaging and Osirix software, and neuroanatomical tracing procedures to study axonal remodeling after stroke and cell transplantation.

Original languageEnglish (US)
Title of host publicationNeural Progenitor Cells
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
Pages157-167
Number of pages11
ISBN (Print)9781627035736
DOIs
StatePublished - 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1059
ISSN (Print)1064-3745

Keywords

  • Cell therapy
  • Neural stem cells
  • Stroke

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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