TY - JOUR
T1 - Isolation and partial characterization of flagellar tubulin from Marsilea vestita
AU - Ludueña, Richard F.
AU - Myles, Diana Gold
AU - Pfeffer, Thomas A.
N1 - Funding Information:
We thank Dr Peter K. Hepler, Dr Leslie Wilson, and Dr Eric M. Shooter in whose laboratoriesp ortions of this research were carried out. We also thank Mary CarmenR oach for expert technicala ssistance. This researchw as supportedb y ACS Grant CH-4A, USPHS Grants NSO9335,a nd NS4270, NSF Grant PCM 74-1524%A02,a grant from the Jane Cofftn Childs Memorial Fund for Medical Research and an institutional Subgrant from The University of Texas. D. G. M. was supportedb y an NIH postdoctoralf el-lowship. R. F. L. is supported by a grant from the Robert Welch Foundation.
PY - 1980/12
Y1 - 1980/12
N2 - Very little biochemical work has been done on plant cell tubulin. In this study, tubulin was isolated from sperm cell flagella from the fern Marsilea vestita. Electrophoresis of reduced and carboxymethylated Marsilea tubulin on three different gel systems revealed that Marsilea tubulin consisted of α- and β-bands, as do the better-known animal and protist tubulins. Comparison of the electrophoretic mobilities of Marsilea, Aspergillus, Chlamydomonas and bovine tubulin showed that Marsilea tubulin's behavior is unique but is most like that of Chlamydomonas. The effects in vivo of tubulin-specific drugs were also examined. Mitosis of Marsilea microspores was sensitive to colchicine, podophyllotoxin and vinblastine, at concentrations higher than those necessary to obtain comparable effects in animal cells, but equivalent to those which block mitosis in Chlamydomonas. In addition, all three drugs, depending on the stage at which they were administered, either prevented flagellar growth or caused formation of abnormal flagella. It thus appears that Marsilea has microtubules similar in many ways to the better characterized animal microtubules but also demonstrating unique characteristics.
AB - Very little biochemical work has been done on plant cell tubulin. In this study, tubulin was isolated from sperm cell flagella from the fern Marsilea vestita. Electrophoresis of reduced and carboxymethylated Marsilea tubulin on three different gel systems revealed that Marsilea tubulin consisted of α- and β-bands, as do the better-known animal and protist tubulins. Comparison of the electrophoretic mobilities of Marsilea, Aspergillus, Chlamydomonas and bovine tubulin showed that Marsilea tubulin's behavior is unique but is most like that of Chlamydomonas. The effects in vivo of tubulin-specific drugs were also examined. Mitosis of Marsilea microspores was sensitive to colchicine, podophyllotoxin and vinblastine, at concentrations higher than those necessary to obtain comparable effects in animal cells, but equivalent to those which block mitosis in Chlamydomonas. In addition, all three drugs, depending on the stage at which they were administered, either prevented flagellar growth or caused formation of abnormal flagella. It thus appears that Marsilea has microtubules similar in many ways to the better characterized animal microtubules but also demonstrating unique characteristics.
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U2 - 10.1016/0014-4827(80)90025-7
DO - 10.1016/0014-4827(80)90025-7
M3 - Article
C2 - 7449863
AN - SCOPUS:0019122772
VL - 130
SP - 455
EP - 459
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 2
ER -