Isolation and differentiation of self-renewable neural stem cells from marmoset-induced pluripotent stem cells

Hyenjong Hong, Gourav Roy-Choudhury, Jeffrey Kim, Marcel M. Daadi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Neural stem cells (NSCs) are multipotent and self-renewing precursor cells that give rise to all cell types of the central nervous system (CNS). They can be used for modeling CNS in vitro, for developmental studies and for cell replacement therapies. NSCs can be derived from pluripotent stem cells through differentiation using specific growth factors. Nonhuman primates (NHP) are critical preclinical models for translational research. Induced pluripotent stem cells (iPSCs) can be generated from NHP for the purposes of allogenic or autologous cell replacement studies. Here, we describe the derivation of NSCs from NHP iPSCs.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages199-204
Number of pages6
DOIs
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1919
ISSN (Print)1064-3745

Keywords

  • Induced pluripotent stem cells
  • Marmosets
  • Neural stem cells
  • Nonhuman primate

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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