Isolation and characterization of the peptidoglycans from selected gram-positive and gram-negative periodontal pathogens

M. R. Barnard, S. C. Holt

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

The peptidoglycans from several Gram-negative and Gram-positive periodontal pathogens were isolated, purified, and characterized both morphologically and chemically. In addition, the effects of the mureolytic enzymes, lysozyme, M-1 N-acetyl-muramidase, and the AM-3 endopeptidase, on the peptidoglycans were examined. These enzymes were found to be highly effective in the degradation of the purified peptidoglycans; however, a Bacteroides capillus peptidoglycan - protein complex exhibited a greater resistance to these enzymes. Morphologically, the peptidoglycans consisted of large saccular sheets which, when viewed by scanning electron microscopy, contained numerous holes and tears. Chemically, the peptidoglycans consisted of muramic acid, glucosamine, alanine, glutamic acid, and meso-diaminopimelic acid (DAP). One Bacteroides species, Bacteroides gingivalis strain W. contained glycine and LL-DAP, suggestive of an indirectly cross-linked A3γ peptidoglycan.

Original languageEnglish (US)
Pages (from-to)154-160
Number of pages7
JournalCanadian journal of microbiology
Volume31
Issue number2
DOIs
StatePublished - Jan 1 1985

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Genetics

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