Abstract
An adriamycin-resistant human breast tumor cell line MDA-A1(R) was generated by step-wise selection in increasing concentrations of drug from the parent cell line MDA-MB-231. MDA-A1(R) cells grow as loosely attached cell aggregates with a doubling time of 28-32 h; the MDA-MB-231 parent cell line grows as a standard monolayer culture with a 20-h doubling time. The MDA-A1(R) cell line is highly resistant to adriamycin compared to the parent cell line, and is cross-resistant to velban and colchicine suggestive of a multidrug resistance (MDR) phenotype. MDA-A1(R) cells exhibit reduced net adriamycin content as compared to the parent cell line. The MDR-associated P-glycoprotein gene is amplified approximately 10- to 30-fold in MDA-A1(R) cells. P-glycoprotein sequences are overexpressed in the resistant cells and are stable for up to 13 wk after drug removal. Moreover, MDA-A1(R) cells show the presence of very high levels of P-glycoprotein. MDA-A1(R) is thus an in vitro model system to study the mechanism of MDR in human breast cancer.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 621-628 |
| Number of pages | 8 |
| Journal | In Vitro Cellular and Developmental Biology - Animal |
| Volume | 26 |
| Issue number | 6 |
| State | Published - 1990 |
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Keywords
- Adriamycin
- breast tumor cells
- drug-resistance
- growth kinetics
- in vitro
- p170
ASJC Scopus subject areas
- Developmental Biology
- Clinical Biochemistry
- Cell Biology
Cite this
Isolation and characterization of an adriamycin-resistant breast tumor cell line. / Schneider, S. L.; Fuqua, S. A W; Speeg, Kermit V; Tandon, A. K.; McGuire, W. L.
In: In Vitro Cellular and Developmental Biology - Animal, Vol. 26, No. 6, 1990, p. 621-628.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation and characterization of an adriamycin-resistant breast tumor cell line
AU - Schneider, S. L.
AU - Fuqua, S. A W
AU - Speeg, Kermit V
AU - Tandon, A. K.
AU - McGuire, W. L.
PY - 1990
Y1 - 1990
N2 - An adriamycin-resistant human breast tumor cell line MDA-A1(R) was generated by step-wise selection in increasing concentrations of drug from the parent cell line MDA-MB-231. MDA-A1(R) cells grow as loosely attached cell aggregates with a doubling time of 28-32 h; the MDA-MB-231 parent cell line grows as a standard monolayer culture with a 20-h doubling time. The MDA-A1(R) cell line is highly resistant to adriamycin compared to the parent cell line, and is cross-resistant to velban and colchicine suggestive of a multidrug resistance (MDR) phenotype. MDA-A1(R) cells exhibit reduced net adriamycin content as compared to the parent cell line. The MDR-associated P-glycoprotein gene is amplified approximately 10- to 30-fold in MDA-A1(R) cells. P-glycoprotein sequences are overexpressed in the resistant cells and are stable for up to 13 wk after drug removal. Moreover, MDA-A1(R) cells show the presence of very high levels of P-glycoprotein. MDA-A1(R) is thus an in vitro model system to study the mechanism of MDR in human breast cancer.
AB - An adriamycin-resistant human breast tumor cell line MDA-A1(R) was generated by step-wise selection in increasing concentrations of drug from the parent cell line MDA-MB-231. MDA-A1(R) cells grow as loosely attached cell aggregates with a doubling time of 28-32 h; the MDA-MB-231 parent cell line grows as a standard monolayer culture with a 20-h doubling time. The MDA-A1(R) cell line is highly resistant to adriamycin compared to the parent cell line, and is cross-resistant to velban and colchicine suggestive of a multidrug resistance (MDR) phenotype. MDA-A1(R) cells exhibit reduced net adriamycin content as compared to the parent cell line. The MDR-associated P-glycoprotein gene is amplified approximately 10- to 30-fold in MDA-A1(R) cells. P-glycoprotein sequences are overexpressed in the resistant cells and are stable for up to 13 wk after drug removal. Moreover, MDA-A1(R) cells show the presence of very high levels of P-glycoprotein. MDA-A1(R) is thus an in vitro model system to study the mechanism of MDR in human breast cancer.
KW - Adriamycin
KW - breast tumor cells
KW - drug-resistance
KW - growth kinetics
KW - in vitro
KW - p170
UR - http://www.scopus.com/inward/record.url?scp=0025356489&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025356489&partnerID=8YFLogxK
M3 - Article
C2 - 1972704
AN - SCOPUS:0025356489
VL - 26
SP - 621
EP - 628
JO - In Vitro Cellular and Developmental Biology - Plant
JF - In Vitro Cellular and Developmental Biology - Plant
SN - 1054-5476
IS - 6
ER -