Isolation and characterization of an adriamycin-resistant breast tumor cell line

S. L. Schneider, S. A W Fuqua, Kermit V Speeg, A. K. Tandon, W. L. McGuire

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

An adriamycin-resistant human breast tumor cell line MDA-A1(R) was generated by step-wise selection in increasing concentrations of drug from the parent cell line MDA-MB-231. MDA-A1(R) cells grow as loosely attached cell aggregates with a doubling time of 28-32 h; the MDA-MB-231 parent cell line grows as a standard monolayer culture with a 20-h doubling time. The MDA-A1(R) cell line is highly resistant to adriamycin compared to the parent cell line, and is cross-resistant to velban and colchicine suggestive of a multidrug resistance (MDR) phenotype. MDA-A1(R) cells exhibit reduced net adriamycin content as compared to the parent cell line. The MDR-associated P-glycoprotein gene is amplified approximately 10- to 30-fold in MDA-A1(R) cells. P-glycoprotein sequences are overexpressed in the resistant cells and are stable for up to 13 wk after drug removal. Moreover, MDA-A1(R) cells show the presence of very high levels of P-glycoprotein. MDA-A1(R) is thus an in vitro model system to study the mechanism of MDR in human breast cancer.

Original languageEnglish (US)
Pages (from-to)621-628
Number of pages8
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume26
Issue number6
StatePublished - 1990

Fingerprint

Tumor Cell Line
Doxorubicin
Tumors
Cells
Breast Neoplasms
Multiple Drug Resistance
P-Glycoprotein
Cell Line
Vinblastine
Colchicine
Cell culture
Pharmaceutical Preparations
Monolayers
Genes
Phenotype

Keywords

  • Adriamycin
  • breast tumor cells
  • drug-resistance
  • growth kinetics
  • in vitro
  • p170

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Isolation and characterization of an adriamycin-resistant breast tumor cell line. / Schneider, S. L.; Fuqua, S. A W; Speeg, Kermit V; Tandon, A. K.; McGuire, W. L.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 26, No. 6, 1990, p. 621-628.

Research output: Contribution to journalArticle

Schneider, S. L. ; Fuqua, S. A W ; Speeg, Kermit V ; Tandon, A. K. ; McGuire, W. L. / Isolation and characterization of an adriamycin-resistant breast tumor cell line. In: In Vitro Cellular and Developmental Biology - Animal. 1990 ; Vol. 26, No. 6. pp. 621-628.
@article{200b931ffa19417fb111751e18f5482f,
title = "Isolation and characterization of an adriamycin-resistant breast tumor cell line",
abstract = "An adriamycin-resistant human breast tumor cell line MDA-A1(R) was generated by step-wise selection in increasing concentrations of drug from the parent cell line MDA-MB-231. MDA-A1(R) cells grow as loosely attached cell aggregates with a doubling time of 28-32 h; the MDA-MB-231 parent cell line grows as a standard monolayer culture with a 20-h doubling time. The MDA-A1(R) cell line is highly resistant to adriamycin compared to the parent cell line, and is cross-resistant to velban and colchicine suggestive of a multidrug resistance (MDR) phenotype. MDA-A1(R) cells exhibit reduced net adriamycin content as compared to the parent cell line. The MDR-associated P-glycoprotein gene is amplified approximately 10- to 30-fold in MDA-A1(R) cells. P-glycoprotein sequences are overexpressed in the resistant cells and are stable for up to 13 wk after drug removal. Moreover, MDA-A1(R) cells show the presence of very high levels of P-glycoprotein. MDA-A1(R) is thus an in vitro model system to study the mechanism of MDR in human breast cancer.",
keywords = "Adriamycin, breast tumor cells, drug-resistance, growth kinetics, in vitro, p170",
author = "Schneider, {S. L.} and Fuqua, {S. A W} and Speeg, {Kermit V} and Tandon, {A. K.} and McGuire, {W. L.}",
year = "1990",
language = "English (US)",
volume = "26",
pages = "621--628",
journal = "In Vitro Cellular and Developmental Biology - Plant",
issn = "1054-5476",
publisher = "Springer New York",
number = "6",

}

TY - JOUR

T1 - Isolation and characterization of an adriamycin-resistant breast tumor cell line

AU - Schneider, S. L.

AU - Fuqua, S. A W

AU - Speeg, Kermit V

AU - Tandon, A. K.

AU - McGuire, W. L.

PY - 1990

Y1 - 1990

N2 - An adriamycin-resistant human breast tumor cell line MDA-A1(R) was generated by step-wise selection in increasing concentrations of drug from the parent cell line MDA-MB-231. MDA-A1(R) cells grow as loosely attached cell aggregates with a doubling time of 28-32 h; the MDA-MB-231 parent cell line grows as a standard monolayer culture with a 20-h doubling time. The MDA-A1(R) cell line is highly resistant to adriamycin compared to the parent cell line, and is cross-resistant to velban and colchicine suggestive of a multidrug resistance (MDR) phenotype. MDA-A1(R) cells exhibit reduced net adriamycin content as compared to the parent cell line. The MDR-associated P-glycoprotein gene is amplified approximately 10- to 30-fold in MDA-A1(R) cells. P-glycoprotein sequences are overexpressed in the resistant cells and are stable for up to 13 wk after drug removal. Moreover, MDA-A1(R) cells show the presence of very high levels of P-glycoprotein. MDA-A1(R) is thus an in vitro model system to study the mechanism of MDR in human breast cancer.

AB - An adriamycin-resistant human breast tumor cell line MDA-A1(R) was generated by step-wise selection in increasing concentrations of drug from the parent cell line MDA-MB-231. MDA-A1(R) cells grow as loosely attached cell aggregates with a doubling time of 28-32 h; the MDA-MB-231 parent cell line grows as a standard monolayer culture with a 20-h doubling time. The MDA-A1(R) cell line is highly resistant to adriamycin compared to the parent cell line, and is cross-resistant to velban and colchicine suggestive of a multidrug resistance (MDR) phenotype. MDA-A1(R) cells exhibit reduced net adriamycin content as compared to the parent cell line. The MDR-associated P-glycoprotein gene is amplified approximately 10- to 30-fold in MDA-A1(R) cells. P-glycoprotein sequences are overexpressed in the resistant cells and are stable for up to 13 wk after drug removal. Moreover, MDA-A1(R) cells show the presence of very high levels of P-glycoprotein. MDA-A1(R) is thus an in vitro model system to study the mechanism of MDR in human breast cancer.

KW - Adriamycin

KW - breast tumor cells

KW - drug-resistance

KW - growth kinetics

KW - in vitro

KW - p170

UR - http://www.scopus.com/inward/record.url?scp=0025356489&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025356489&partnerID=8YFLogxK

M3 - Article

C2 - 1972704

AN - SCOPUS:0025356489

VL - 26

SP - 621

EP - 628

JO - In Vitro Cellular and Developmental Biology - Plant

JF - In Vitro Cellular and Developmental Biology - Plant

SN - 1054-5476

IS - 6

ER -