Abstract
An adriamycin-resistant human breast tumor cell line MDA-A1(R) was generated by step-wise selection in increasing concentrations of drug from the parent cell line MDA-MB-231. MDA-A1(R) cells grow as loosely attached cell aggregates with a doubling time of 28-32 h; the MDA-MB-231 parent cell line grows as a standard monolayer culture with a 20-h doubling time. The MDA-A1(R) cell line is highly resistant to adriamycin compared to the parent cell line, and is cross-resistant to velban and colchicine suggestive of a multidrug resistance (MDR) phenotype. MDA-A1(R) cells exhibit reduced net adriamycin content as compared to the parent cell line. The MDR-associated P-glycoprotein gene is amplified approximately 10- to 30-fold in MDA-A1(R) cells. P-glycoprotein sequences are overexpressed in the resistant cells and are stable for up to 13 wk after drug removal. Moreover, MDA-A1(R) cells show the presence of very high levels of P-glycoprotein. MDA-A1(R) is thus an in vitro model system to study the mechanism of MDR in human breast cancer.
Original language | English (US) |
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Pages (from-to) | 621-628 |
Number of pages | 8 |
Journal | In Vitro Cellular and Developmental Biology - Animal |
Volume | 26 |
Issue number | 6 |
DOIs | |
State | Published - Jan 1 1990 |
Keywords
- Adriamycin
- breast tumor cells
- drug-resistance
- growth kinetics
- in vitro
- p170
ASJC Scopus subject areas
- Developmental Biology
- Clinical Biochemistry
- Cell Biology