Isolation and characterization of an adriamycin-resistant breast tumor cell line

S. L. Schneider, S. A.W. Fuqua, K. V. Speeg, A. K. Tandon, W. L. McGuire

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

An adriamycin-resistant human breast tumor cell line MDA-A1(R) was generated by step-wise selection in increasing concentrations of drug from the parent cell line MDA-MB-231. MDA-A1(R) cells grow as loosely attached cell aggregates with a doubling time of 28-32 h; the MDA-MB-231 parent cell line grows as a standard monolayer culture with a 20-h doubling time. The MDA-A1(R) cell line is highly resistant to adriamycin compared to the parent cell line, and is cross-resistant to velban and colchicine suggestive of a multidrug resistance (MDR) phenotype. MDA-A1(R) cells exhibit reduced net adriamycin content as compared to the parent cell line. The MDR-associated P-glycoprotein gene is amplified approximately 10- to 30-fold in MDA-A1(R) cells. P-glycoprotein sequences are overexpressed in the resistant cells and are stable for up to 13 wk after drug removal. Moreover, MDA-A1(R) cells show the presence of very high levels of P-glycoprotein. MDA-A1(R) is thus an in vitro model system to study the mechanism of MDR in human breast cancer.

Original languageEnglish (US)
Pages (from-to)621-628
Number of pages8
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume26
Issue number6
DOIs
StatePublished - Jan 1 1990

Keywords

  • Adriamycin
  • breast tumor cells
  • drug-resistance
  • growth kinetics
  • in vitro
  • p170

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

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