Isolation and characterization of a hemin-binding cell envelope protein from Porphyromonas gingivalis

Sung Jo Kim; L. Chu; S. C. Holt

doi:10.1006/mpat.1996.0043

Isolation and characterization of a hemin-binding cell envelope protein from Porphyromonas gingivalis

Sung Jo Kim, L. Chu, S. C. Holt

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

A 30 kDa (heated 24 kDa) hemin-binding protein whose expression is both hemin and iron regulated was identified and purified in Porphyromonas gingivalis 381. A strong hemin-binding function was found by LDS-PAGE and TMBZ staining when cells were grown under hemin (iron)-limited conditions. N-terminal amino acid sequence analysis of CNBr-digested 24 kDa hemin binding protein revealed that this protein belongs to a new, so far undescribed hemin-binding class of proteins.

Original language	English (US)
Pages (from-to)	65-70
Number of pages	6
Journal	Microbial Pathogenesis
Volume	21
Issue number	1
DOIs	https://doi.org/10.1006/mpat.1996.0043
State	Published - Jul 1996

Keywords

Hemin
Outer membranes
Porphyromonas gingivalis
TMBZ

ASJC Scopus subject areas

Microbiology
Infectious Diseases

Access to Document

10.1006/mpat.1996.0043

Cite this

@article{5fc454332cc3424caa369fbcc08c8f55,

title = "Isolation and characterization of a hemin-binding cell envelope protein from Porphyromonas gingivalis",

abstract = "A 30 kDa (heated 24 kDa) hemin-binding protein whose expression is both hemin and iron regulated was identified and purified in Porphyromonas gingivalis 381. A strong hemin-binding function was found by LDS-PAGE and TMBZ staining when cells were grown under hemin (iron)-limited conditions. N-terminal amino acid sequence analysis of CNBr-digested 24 kDa hemin binding protein revealed that this protein belongs to a new, so far undescribed hemin-binding class of proteins.",

keywords = "Hemin, Outer membranes, Porphyromonas gingivalis, TMBZ",

author = "Kim, {Sung Jo} and L. Chu and Holt, {S. C.}",

note = "Funding Information: This work was supported by USPHS grant DE-07627 from the National Institute of Dental Research.",

year = "1996",

month = jul,

doi = "10.1006/mpat.1996.0043",

language = "English (US)",

volume = "21",

pages = "65--70",

journal = "Microbial Pathogenesis",

issn = "0882-4010",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Isolation and characterization of a hemin-binding cell envelope protein from Porphyromonas gingivalis

AU - Kim, Sung Jo

AU - Chu, L.

AU - Holt, S. C.

N1 - Funding Information: This work was supported by USPHS grant DE-07627 from the National Institute of Dental Research.

PY - 1996/7

Y1 - 1996/7

N2 - A 30 kDa (heated 24 kDa) hemin-binding protein whose expression is both hemin and iron regulated was identified and purified in Porphyromonas gingivalis 381. A strong hemin-binding function was found by LDS-PAGE and TMBZ staining when cells were grown under hemin (iron)-limited conditions. N-terminal amino acid sequence analysis of CNBr-digested 24 kDa hemin binding protein revealed that this protein belongs to a new, so far undescribed hemin-binding class of proteins.

AB - A 30 kDa (heated 24 kDa) hemin-binding protein whose expression is both hemin and iron regulated was identified and purified in Porphyromonas gingivalis 381. A strong hemin-binding function was found by LDS-PAGE and TMBZ staining when cells were grown under hemin (iron)-limited conditions. N-terminal amino acid sequence analysis of CNBr-digested 24 kDa hemin binding protein revealed that this protein belongs to a new, so far undescribed hemin-binding class of proteins.

KW - Hemin

KW - Outer membranes

KW - Porphyromonas gingivalis

KW - TMBZ

UR - http://www.scopus.com/inward/record.url?scp=0030200413&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030200413&partnerID=8YFLogxK

U2 - 10.1006/mpat.1996.0043

DO - 10.1006/mpat.1996.0043

M3 - Article

C2 - 8827708

AN - SCOPUS:0030200413

VL - 21

SP - 65

EP - 70

JO - Microbial Pathogenesis

JF - Microbial Pathogenesis

SN - 0882-4010

IS - 1

ER -

Isolation and characterization of a hemin-binding cell envelope protein from Porphyromonas gingivalis

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this