Abstract
A 30 kDa (heated 24 kDa) hemin-binding protein whose expression is both hemin and iron regulated was identified and purified in Porphyromonas gingivalis 381. A strong hemin-binding function was found by LDS-PAGE and TMBZ staining when cells were grown under hemin (iron)-limited conditions. N-terminal amino acid sequence analysis of CNBr-digested 24 kDa hemin binding protein revealed that this protein belongs to a new, so far undescribed hemin-binding class of proteins.
Original language | English (US) |
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Pages (from-to) | 65-70 |
Number of pages | 6 |
Journal | Microbial Pathogenesis |
Volume | 21 |
Issue number | 1 |
DOIs | |
State | Published - Jul 1996 |
Keywords
- Hemin
- Outer membranes
- Porphyromonas gingivalis
- TMBZ
ASJC Scopus subject areas
- Microbiology
- Infectious Diseases