Isolation and characterization of a hemin-binding cell envelope protein from Porphyromonas gingivalis

Sung Jo Kim; L. Chu; S. C. Holt

doi:10.1006/mpat.1996.0043

Isolation and characterization of a hemin-binding cell envelope protein from Porphyromonas gingivalis

Sung Jo Kim, L. Chu, S. C. Holt

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

A 30 kDa (heated 24 kDa) hemin-binding protein whose expression is both hemin and iron regulated was identified and purified in Porphyromonas gingivalis 381. A strong hemin-binding function was found by LDS-PAGE and TMBZ staining when cells were grown under hemin (iron)-limited conditions. N-terminal amino acid sequence analysis of CNBr-digested 24 kDa hemin binding protein revealed that this protein belongs to a new, so far undescribed hemin-binding class of proteins.

Original language	English (US)
Pages (from-to)	65-70
Number of pages	6
Journal	Microbial Pathogenesis
Volume	21
Issue number	1
DOIs	https://doi.org/10.1006/mpat.1996.0043
State	Published - Jul 1996

Keywords

Hemin
Outer membranes
Porphyromonas gingivalis
TMBZ

ASJC Scopus subject areas

Microbiology
Infectious Diseases

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10.1006/mpat.1996.0043

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title = "Isolation and characterization of a hemin-binding cell envelope protein from Porphyromonas gingivalis",

abstract = "A 30 kDa (heated 24 kDa) hemin-binding protein whose expression is both hemin and iron regulated was identified and purified in Porphyromonas gingivalis 381. A strong hemin-binding function was found by LDS-PAGE and TMBZ staining when cells were grown under hemin (iron)-limited conditions. N-terminal amino acid sequence analysis of CNBr-digested 24 kDa hemin binding protein revealed that this protein belongs to a new, so far undescribed hemin-binding class of proteins.",

keywords = "Hemin, Outer membranes, Porphyromonas gingivalis, TMBZ",

author = "Kim, {Sung Jo} and L. Chu and Holt, {S. C.}",

note = "Funding Information: This work was supported by USPHS grant DE-07627 from the National Institute of Dental Research.",

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AU - Chu, L.

AU - Holt, S. C.

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AB - A 30 kDa (heated 24 kDa) hemin-binding protein whose expression is both hemin and iron regulated was identified and purified in Porphyromonas gingivalis 381. A strong hemin-binding function was found by LDS-PAGE and TMBZ staining when cells were grown under hemin (iron)-limited conditions. N-terminal amino acid sequence analysis of CNBr-digested 24 kDa hemin binding protein revealed that this protein belongs to a new, so far undescribed hemin-binding class of proteins.

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KW - Outer membranes

KW - Porphyromonas gingivalis

KW - TMBZ

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