Isolation and characterization of a hemin-binding cell envelope protein from Porphyromonas gingivalis

Sung Jo Kim, L. Chu, S. C. Holt

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

A 30 kDa (heated 24 kDa) hemin-binding protein whose expression is both hemin and iron regulated was identified and purified in Porphyromonas gingivalis 381. A strong hemin-binding function was found by LDS-PAGE and TMBZ staining when cells were grown under hemin (iron)-limited conditions. N-terminal amino acid sequence analysis of CNBr-digested 24 kDa hemin binding protein revealed that this protein belongs to a new, so far undescribed hemin-binding class of proteins.

Original languageEnglish (US)
Pages (from-to)65-70
Number of pages6
JournalMicrobial Pathogenesis
Volume21
Issue number1
DOIs
StatePublished - Jul 1996

Keywords

  • Hemin
  • Outer membranes
  • Porphyromonas gingivalis
  • TMBZ

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

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