Is there a risk of cytomegalovirus transmission during in vitro fertilization with donated oocytes?

Craig A. Witz, Yanping Duan, William N. Burns, Sally S. Atherton, Robert S. Schenken

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Objective: To define the risk of human cytomegalovirus (HCMV) transmission from donated oocytes. Design: Prospective study. Setting: University IVF program. Patient(s): Sixty-seven couples undergoing 72 cycles of IVF-ET. Intervention(s): None. Main Outcome Measure(s): Serum from both partners (women: n = 71; men: n = 60) was obtained for detection of antibodies to HCMV. Semen before preparation (n = 53), sperm after preparation (Percoll gradient; n = 47), cervical mucus aspirated at the time of oocyte aspiration (n = 70), and uninseminated oocytes and embryos not suitable for cryopreservation (n = 568) were frozen in liquid nitrogen. Polymerase chain reaction was used for detection of HCMV (immediate early 1 gene) in all samples collected. Result(s): Serum antibodies to HCMV were found in 62% of the women and 37% of the men tested. Human cytomegalovirus DNA was detected in 25% of the ejaculates and in 19% of the cervical mucus samples. There was no amplification of HCMV DNA from oocytes or embryos. Conclusion(s): Because we were unable to amplify HCMV DNA from any of the oocytes or embryos, it seems unlikely that HCMV is transmissible through oocyte or embryo donation.

Original languageEnglish (US)
Pages (from-to)302-307
Number of pages6
JournalFertility and Sterility
Volume71
Issue number2
DOIs
StatePublished - Feb 1 1999

Keywords

  • Cytomegalovirus
  • Donation
  • In vitro fertilization
  • Oocyte
  • Polymerase chain reaction
  • Sperm

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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