TY - JOUR
T1 - Iron-regulated outer membrane proteins in the periodontopathic bacterium, Bacteroides gingivalis
AU - Bramanti, Thomas E.
AU - Holt, Stanley C.
N1 - Funding Information:
This study was supported in part by PHS grants DE-00152, DE-07267, and DE-08569. We thank Daniel Guerrero for his photographic su port and W. Kennell, A. Weinberg, and S. Kinder for continual interest and discussion oft RI S work.
PY - 1990/2/14
Y1 - 1990/2/14
N2 - Hemin has been implicated in the pathogenesis of the oral pathogen, Bacteroides gingivalis. In order to elucidate the role of hemin (iron) in the growth and expression of outer membrane proteins, B. gingivalis strain W50 was grown with and without hemin to induce iron-limitation. Cells grew slower under iron stress and growth was completely inhibited in the absence of added hemin. The outer membrane protein profiles of B. gingivalis grown under iron-replete and iron-restricted conditions were studied by extrinsic radiolabelling with [125I] and polyacrylamide gel-electrophoresis. The induction of 10 surface proteins, with apparent molecular weights of 26, 29, 50, 56, 58, 60, 62, 71, 77, and 80 Kd, was observed in B. gingivalis grown under iron-restricted conditions. These proteins were repressed under iron-replete conditions. We postulate the involvement of the iron-regulated proteins in hemin uptake and virulence in B.gingivalis.
AB - Hemin has been implicated in the pathogenesis of the oral pathogen, Bacteroides gingivalis. In order to elucidate the role of hemin (iron) in the growth and expression of outer membrane proteins, B. gingivalis strain W50 was grown with and without hemin to induce iron-limitation. Cells grew slower under iron stress and growth was completely inhibited in the absence of added hemin. The outer membrane protein profiles of B. gingivalis grown under iron-replete and iron-restricted conditions were studied by extrinsic radiolabelling with [125I] and polyacrylamide gel-electrophoresis. The induction of 10 surface proteins, with apparent molecular weights of 26, 29, 50, 56, 58, 60, 62, 71, 77, and 80 Kd, was observed in B. gingivalis grown under iron-restricted conditions. These proteins were repressed under iron-replete conditions. We postulate the involvement of the iron-regulated proteins in hemin uptake and virulence in B.gingivalis.
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U2 - 10.1016/0006-291X(90)90986-W
DO - 10.1016/0006-291X(90)90986-W
M3 - Article
C2 - 2306235
AN - SCOPUS:0025174725
VL - 166
SP - 1146
EP - 1154
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -