Iodination by human polymorphonuclear leukocytes: a re-evaluation

Seymour J. Klebanoff, Robert A. Clark

Research output: Contribution to journalArticlepeer-review

78 Scopus citations


The conversion of iodide to a trichloroacetic acid-precipitable form (iodination) by polymorphonuclear leukocytes (PMN's) has been re-evaluated as a measure of neutrophil function. Optimum conditions are described which result in an iodination value for normal cells during the phagocytosis of zymosan of 64.1 ± 13.2 (S.D.) nmol. per 107 PMN's per hour. Iodination is inhibited by agents which decrease phagocytosis, inhibit myeloperoxidase-catalyzed reactions, or degrade H2O2 and is stimulated by superoxide dismutase, an enzyme which catalyzes the conversion of the superoxide anion to oxygen and H2O2. When patients' cells and normal serum are employed, the iodinating capacity of the patients' cells is evaluated. It is low in patients with myeloperoxidase deficiency and chronic granulomatous disease, and an intermediate value was observed in a carrier of chronic granulomatous disease. When normal cells and patients' serum are employed, the iodination reaction is an indirect measure of the opsonic activity of the patients' serum. The decreased opsonic activity for zymosan of human sera deficient in the fourth or third component of complement was demonstrated in this way. Thus measurement of iodination is a convenient and sensitive screening test for cellular or humoral abnormalities of the phagocytic process.

Original languageEnglish (US)
Pages (from-to)675-686
Number of pages12
JournalThe Journal of Laboratory and Clinical Medicine
Issue number3
StatePublished - Mar 1977
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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