Involvement of CNOT3 in mitotic progression through inhibition of MAD1 expression

Akinori Takahashi, Chisato Kikuguchi, Masahiro Morita, Tetsuhiro Shimodaira, Noriko Tokai-Nishizumi, Kazumasa Yokoyama, Miho Ohsugi, Toru Suzuki, Tadashi Yamamoto

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The stability of mRNA influences the dynamics of gene expression. The CCR4-NOT complex, the major deadenylase in mammalian cells, shortens the mRNA poly(A) tail and contributes to the destabilization of mRNAs. The CCR4-NOT complex plays pivotal roles in various physiological functions, including cell proliferation, apoptosis, and metabolism. Here, we show that CNOT3, a subunit of the CCR4-NOT complex, is involved in the regulation of the spindle assembly checkpoint, suggesting that the CCR4-NOT complex also plays a part in the regulation of mitosis. CNOT3 depletion increases the population of mitotic-arrested cells and specifically increases the expression of . MAD1 mRNA and its protein product that plays a part in the spindle assembly checkpoint. We showed that CNOT3 depletion stabilizes the . MAD1 mRNA, and that . MAD1 knockdown attenuates the CNOT3 depletion-induced increase of the mitotic index. Basing on these observations, we propose that CNOT3 is involved in the regulation of the spindle assembly checkpoint through its ability to regulate the stability of . MAD1 mRNA.

Original languageEnglish (US)
Pages (from-to)268-273
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume419
Issue number2
DOIs
StatePublished - Mar 9 2012
Externally publishedYes

Fingerprint

M Phase Cell Cycle Checkpoints
Messenger RNA
Cells
Mitotic Index
RNA Stability
Cell proliferation
Mitosis
Metabolism
Gene expression
Cell Proliferation
Apoptosis
Gene Expression
Population
Proteins

Keywords

  • CCR4-NOT complex
  • CNOT3
  • MAD1
  • Mitosis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Takahashi, A., Kikuguchi, C., Morita, M., Shimodaira, T., Tokai-Nishizumi, N., Yokoyama, K., ... Yamamoto, T. (2012). Involvement of CNOT3 in mitotic progression through inhibition of MAD1 expression. Biochemical and Biophysical Research Communications, 419(2), 268-273. https://doi.org/10.1016/j.bbrc.2012.02.007

Involvement of CNOT3 in mitotic progression through inhibition of MAD1 expression. / Takahashi, Akinori; Kikuguchi, Chisato; Morita, Masahiro; Shimodaira, Tetsuhiro; Tokai-Nishizumi, Noriko; Yokoyama, Kazumasa; Ohsugi, Miho; Suzuki, Toru; Yamamoto, Tadashi.

In: Biochemical and Biophysical Research Communications, Vol. 419, No. 2, 09.03.2012, p. 268-273.

Research output: Contribution to journalArticle

Takahashi, A, Kikuguchi, C, Morita, M, Shimodaira, T, Tokai-Nishizumi, N, Yokoyama, K, Ohsugi, M, Suzuki, T & Yamamoto, T 2012, 'Involvement of CNOT3 in mitotic progression through inhibition of MAD1 expression', Biochemical and Biophysical Research Communications, vol. 419, no. 2, pp. 268-273. https://doi.org/10.1016/j.bbrc.2012.02.007
Takahashi, Akinori ; Kikuguchi, Chisato ; Morita, Masahiro ; Shimodaira, Tetsuhiro ; Tokai-Nishizumi, Noriko ; Yokoyama, Kazumasa ; Ohsugi, Miho ; Suzuki, Toru ; Yamamoto, Tadashi. / Involvement of CNOT3 in mitotic progression through inhibition of MAD1 expression. In: Biochemical and Biophysical Research Communications. 2012 ; Vol. 419, No. 2. pp. 268-273.
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