TY - JOUR
T1 - Investigation of DNA Loop Domains using Fluorescent in Situ Hybridization (FISH) and Epifluorescence Microscopy
AU - Klaus, Angela V.
AU - McCarthy, Siobhan
AU - McCarrey, John
AU - Ward, W. Steven
N1 - Publisher Copyright:
© 1998 Microscopy Society of America.
PY - 1998/7/1
Y1 - 1998/7/1
N2 - The average sperm nucleus is only 8 micrometers long, but contains 1 meter of DNA. We have previously described how the sperm cell is able to package this DNA into the small volume of the sperm head. The only DNA structure that exists in both somatic cells and sperm nuclei is the organization of DNA loop domains that are attached at their bases to the nuclear skeleton, the nuclear matrix. We compared the structure of one gene in sperm nuclei to that of spermatogenic and adult cells. Individual loop domains of the 5S rRNA gene cluster were visualized by fluorescent in situ hybridization (FISH). Nuclei were isolated from hamster spermatozoa, spermatogonia, pachytene spermatocytes, round spermatids, and adult liver cells. They were then extracted to remove the protamines or histones, fixed, hybridized to a biotinylated 5S rDNA probe, then viewed by epifluorescence microscopy.
AB - The average sperm nucleus is only 8 micrometers long, but contains 1 meter of DNA. We have previously described how the sperm cell is able to package this DNA into the small volume of the sperm head. The only DNA structure that exists in both somatic cells and sperm nuclei is the organization of DNA loop domains that are attached at their bases to the nuclear skeleton, the nuclear matrix. We compared the structure of one gene in sperm nuclei to that of spermatogenic and adult cells. Individual loop domains of the 5S rRNA gene cluster were visualized by fluorescent in situ hybridization (FISH). Nuclei were isolated from hamster spermatozoa, spermatogonia, pachytene spermatocytes, round spermatids, and adult liver cells. They were then extracted to remove the protamines or histones, fixed, hybridized to a biotinylated 5S rDNA probe, then viewed by epifluorescence microscopy.
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U2 - 10.1017/S1431927600025708
DO - 10.1017/S1431927600025708
M3 - Article
AN - SCOPUS:85180323170
SN - 1431-9276
VL - 4
SP - 1116
EP - 1117
JO - Microscopy and Microanalysis
JF - Microscopy and Microanalysis
ER -