Investigation of cellular adhesiveness as a function of substrate using cytodetachment technique

K. A. Athanasiou, D. Shin, B. Thoma, C. M. Agrawal, R. G. Lebaron

Research output: Contribution to conferencePaper

Abstract

Bovine chondrocytes were seeded on glass slides which are coated with bovine serum albumin and fibronectin for comparison. Following cell attachment for 24 hours, the attachment forces of bovine chondrocyte were measured using the newly designed cytodetachment apparatus. By manipulating the microscope stage and the instrument's six-degree-of-freedom assembly, the cytodetachment probe approaches the attached cell from its side. It was found that adhesion of cells to the foreign surface is a necessary prerequisite to facilitate focal adhesions and cell proliferation. Furthermore, the changes in cell adhesiveness that were observed is due to the intrinsic properties of cells, as modulated by different substrates.

Original languageEnglish (US)
Number of pages1
StatePublished - Jan 1 1998
EventProceedings of the 1998 17th Southern Biomedical Engineering Conference - San Antonio, TX, USA
Duration: Feb 6 1998Feb 8 1998

Other

OtherProceedings of the 1998 17th Southern Biomedical Engineering Conference
CitySan Antonio, TX, USA
Period2/6/982/8/98

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ASJC Scopus subject areas

  • Engineering(all)

Cite this

Athanasiou, K. A., Shin, D., Thoma, B., Agrawal, C. M., & Lebaron, R. G. (1998). Investigation of cellular adhesiveness as a function of substrate using cytodetachment technique. Paper presented at Proceedings of the 1998 17th Southern Biomedical Engineering Conference, San Antonio, TX, USA, .