Investigation of cellular adhesiveness as a function of substrate using cytodetachment technique

K. A. Athanasiou; D. Shin; B. Thoma; C. M. Agrawal; R. G. Lebaron

Investigation of cellular adhesiveness as a function of substrate using cytodetachment technique

K. A. Athanasiou, D. Shin, B. Thoma, C. M. Agrawal, R. G. Lebaron

Orthopaedics

Research output: Contribution to conference › Paper › peer-review

Abstract

Bovine chondrocytes were seeded on glass slides which are coated with bovine serum albumin and fibronectin for comparison. Following cell attachment for 24 hours, the attachment forces of bovine chondrocyte were measured using the newly designed cytodetachment apparatus. By manipulating the microscope stage and the instrument's six-degree-of-freedom assembly, the cytodetachment probe approaches the attached cell from its side. It was found that adhesion of cells to the foreign surface is a necessary prerequisite to facilitate focal adhesions and cell proliferation. Furthermore, the changes in cell adhesiveness that were observed is due to the intrinsic properties of cells, as modulated by different substrates.

Original language	English (US)
Number of pages	1
State	Published - 1998
Event	Proceedings of the 1998 17th Southern Biomedical Engineering Conference - San Antonio, TX, USA Duration: Feb 6 1998 → Feb 8 1998

Other

Other	Proceedings of the 1998 17th Southern Biomedical Engineering Conference
City	San Antonio, TX, USA
Period	2/6/98 → 2/8/98

ASJC Scopus subject areas

Engineering(all)

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title = "Investigation of cellular adhesiveness as a function of substrate using cytodetachment technique",

abstract = "Bovine chondrocytes were seeded on glass slides which are coated with bovine serum albumin and fibronectin for comparison. Following cell attachment for 24 hours, the attachment forces of bovine chondrocyte were measured using the newly designed cytodetachment apparatus. By manipulating the microscope stage and the instrument's six-degree-of-freedom assembly, the cytodetachment probe approaches the attached cell from its side. It was found that adhesion of cells to the foreign surface is a necessary prerequisite to facilitate focal adhesions and cell proliferation. Furthermore, the changes in cell adhesiveness that were observed is due to the intrinsic properties of cells, as modulated by different substrates.",

author = "Athanasiou, {K. A.} and D. Shin and B. Thoma and Agrawal, {C. M.} and Lebaron, {R. G.}",

year = "1998",

language = "English (US)",

note = "Proceedings of the 1998 17th Southern Biomedical Engineering Conference ; Conference date: 06-02-1998 Through 08-02-1998",

}

TY - CONF

T1 - Investigation of cellular adhesiveness as a function of substrate using cytodetachment technique

AU - Athanasiou, K. A.

AU - Shin, D.

AU - Thoma, B.

AU - Agrawal, C. M.

AU - Lebaron, R. G.

PY - 1998

Y1 - 1998

N2 - Bovine chondrocytes were seeded on glass slides which are coated with bovine serum albumin and fibronectin for comparison. Following cell attachment for 24 hours, the attachment forces of bovine chondrocyte were measured using the newly designed cytodetachment apparatus. By manipulating the microscope stage and the instrument's six-degree-of-freedom assembly, the cytodetachment probe approaches the attached cell from its side. It was found that adhesion of cells to the foreign surface is a necessary prerequisite to facilitate focal adhesions and cell proliferation. Furthermore, the changes in cell adhesiveness that were observed is due to the intrinsic properties of cells, as modulated by different substrates.

AB - Bovine chondrocytes were seeded on glass slides which are coated with bovine serum albumin and fibronectin for comparison. Following cell attachment for 24 hours, the attachment forces of bovine chondrocyte were measured using the newly designed cytodetachment apparatus. By manipulating the microscope stage and the instrument's six-degree-of-freedom assembly, the cytodetachment probe approaches the attached cell from its side. It was found that adhesion of cells to the foreign surface is a necessary prerequisite to facilitate focal adhesions and cell proliferation. Furthermore, the changes in cell adhesiveness that were observed is due to the intrinsic properties of cells, as modulated by different substrates.

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M3 - Paper

AN - SCOPUS:0031650567

T2 - Proceedings of the 1998 17th Southern Biomedical Engineering Conference

Y2 - 6 February 1998 through 8 February 1998

ER -